LOCUS       sequence-124943-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild type SpCas9 expression plasmid to clone TALEs through BbsI site
            (generates compatible overhangs with Acc65I and BamHI sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..40
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             104..130
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
ORIGIN
        1 gggtagagcc gcgccggcag ctaagaaaaa gaaactggat caattgggcg ctagcggtag
       61 tcccgggtct ggaggtagcg gctcaagtgg ccgtacggca gctcctgcgg caaaaaagaa
      121 aaagttggac tctgaattcg gaagcggaag cgacaagaag tactccattg ggctcgatat
      181 cggtaccaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa
      241 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggagccct
      301 cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag
      361 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
      421 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
      481 aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa
      541 gtacccaacc atatatcatc tgaggaagaa gctggtagac agtactgata aggctgactt
      601 gcggttgatc tatctcgcgc tggcgcacat gatcaaattt cggggacact tcctcatcga
      661 gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac
      721 tta
//
