LOCUS       sequence-124947-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V2 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-278
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             100..123
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             124..144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattgatta attcgagcga acgcgtatgg actacaagga cgacgatgac
      121 aagcccaaga aaaagcgcaa ggtctctaga cccggggagc gccccttcca gtgtcgcatt
      181 tgcatgcgga acttttcgga taaaactaaa ttgagagttc atacccgtac tcataccggt
      241 gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct ccgttagaca taatttgact
      301 agacatctac gtacgcacac cggcgagaag ccattccaat gccgaatatg catgcgcaac
      361 ttcagtcaat ctacttcttt gcaaagacac ctaaaaaccc acctgagagg attcggcgga
      421 gtgctggaga agggctgctt tgccaagggt accaatgttt taatggcgga tgggtctatt
      481 gaatgtattg aaaacattga ggttggtaat aaggtcatgg gtaaagatgg cagacctcgt
      541 gaggtaatta aattgcccag aggaagagaa actatgtaca gcgtcgtgca gaaaagtcag
      601 cacagagccc ac
//
