LOCUS       sequence-124948-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V2 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-278
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    124..145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(160..165,166..183,184..190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    198..214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     203..225
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     222..238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     222..238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     581..600
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    599..654
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     700..719
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 tctcccgcgc gttggccgat tcattaatgc agcggatctc tcgagttaat ttcgcttaat
       61 ttcgcggatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      121 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      181 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      241 tgattacgcc aagcttgggc tgcaggtcga gggatctcca taagagaaga gggacagcta
      301 tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt
      361 ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag
      421 tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac
      481 ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt gccaaactct
      541 aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc cttccgagtg
      601 agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag
      661 aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat
      721 ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc tgataggcag
      781 cctgc
//
