LOCUS       sequence-124950-         273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V2m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-279
FEATURES             Location/Qualifiers
     source          1..273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattgattaa ttcgagcgaa cgcgtatgga ctacaaggac gacgatgaca agcccaagaa
       61 aaagcgcaag gtctctagac ccggggagcg ccccttccag tgtcgcattt gcatgcggaa
      121 cttttcggat aaaactaaat tgagagttca tacccgtact cataccggtg aaaaaccgtt
      181 tcagtgtcgg atctgtatgc gaaatttctc cgttagacat aatttgacta gacatctacg
      241 tacgcacacc ggcgagaagc cattccaatg ccg
//
