LOCUS       sequence-124953-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-280
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tgattaattc gagcgaacgc gtatggacta caaggacgac
      121 gatgacaagc ccaagaaaaa gcgcaaggtc tctagacccg gggagcgccc cttccagtgt
      181 cgcatttgca tgcggaactt ttcggataaa actaaattga gagttcatac ccgtactcat
      241 accggtgaaa aaccgtttca gtgtcggatc tgtatgcgaa atttctccgt tagacataat
      301 ttgactagac atctacgtac gcacaccggc gagaagccat tccaatgccg aatatgcatg
      361 cgcaacttca gtcaatctac ttctttgcaa agacacctaa aaacccacct gagaggatcc
      421 ggcggaggct ggagactggg ctgctttgcc aagggtacca atgttttaat ggcggatggg
      481 tctattgaat gtattgaaaa cattgaggtt ggtaataagg tcatgggtaa agatggcaga
      541 cctcgtgagg taattaaatt gcccagagga agagaaacta tgtacagcgt cgtgcagaaa
      601 agtcag
//
