LOCUS       sequence-124954-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-280
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(126..131,132..149,150..156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     169..191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     188..204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     188..204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     547..566
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    565..620
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     666..685
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 atctctcgag ttaatttcgc ttaatttcgc ggatccgcat ggcacgacag gtttcccgac
       61 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      181 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
      241 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
      301 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
      361 aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc
      421 agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag
      481 catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
      541 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat
      601 gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc
      661 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
      721 accagccacc accttctgat aggcagcctg cacctgagga gtgcggccgc tt
//
