LOCUS       sequence-124956-         448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-281
FEATURES             Location/Qualifiers
     source          1..448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             64..84
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttggcaaa gaattgatta attcgagcga acgcgtatgg actacaagga cgacgatgac
       61 aagcccaaga aaaagcgcaa ggtctctaga cccggggagc gccccttcca gtgtcgcatt
      121 tgcatgcgga acttttcgga taaaactaaa ttgagagttc atacccgtac tcataccggt
      181 gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct ccgttagaca taatttgact
      241 agacatctac gtacgcacac cggcgagaag ccattccaat gccgaatatg catgcgcaac
      301 ttcagtcaat ctacttcttt gcaaagacac ctaaaaaccc acctgagagg atccggcgga
      361 ggctggagac tgggcgcctt tgccaagggt accaatgttt taatggcgga tgggtctatt
      421 gaatgtattg aaaacattga ggttggta
//
