LOCUS       sequence-124957-         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-281
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..63
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(78..83,84..101,102..108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    116..132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     121..143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     140..156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     140..156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     499..518
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
       61 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      121 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg
      181 caggtcnagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
      241 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt anggatgtta aagaaaaaaa
      301 taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa
      361 ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt
      421 catggcagcc agcatatggg catatgttgc caaactctaa accaaatact cattctgatg
      481 ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaa
//
