LOCUS       sequence-124964-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter with wild-type qacR on single plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES005 (pTet-qacR wt/pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             118..129
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 catccacgca ttataacgtc gtattagctg cctttcgtct tcaataattc ttgacaccct
       61 atcagtgata gagatacttc gtgcaatttt tatatcaccg ccaggggtac aacaagcaaa
      121 gaggagaaat actagcatga acttgaaaga taagattttg ggtgttgcta aagagctgtt
      181 tatcaaaaac ggttacaatg ctactaccac tggtgagatt gtgaaattgt ctgaatcttc
      241 taaagggaat ctatactatc atttcaaaac gaaggaaaat ttgttcttgg aaatcttgaa
      301 cattgaagag agtaaatggc aagaacaatg gaaaaaggag cagattaaag ctaaaactaa
      361 tagagaaaag ttttatctgt ataatgagtt gtctttgact acagagtatt actatccact
      421 acagaacgca ataatcgagt tctacactga atactataag actaactcca tcaatgaaaa
      481 aatgaacaaa ttagagaata agtatatcga tgcatatcac gttattttca acgaaggtaa
      541 tcttaacggt gagtggagta taaatgatgt taatgccgtt tctaaaattg ctgctaacgc
      601 tgttaacggt attgttacat tcacacatga g
//
