LOCUS       sequence-124970-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter with qacR 5 on single plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES007 (pTet-qacR 5/pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             174..185
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             766..783
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttccgat ggcgcgccga gaggctttac actttatgct tccggctggt ctcagcatcc
       61 acgcattata acgtcgtatt agctgccttt cgtcttcaat aattcttgac atccctatca
      121 gtgataggga tacttcgtgc aatttttata tcaccgccag gggtacaaca agcaaagagg
      181 agaaatacta gcatgaactt gaaagataag attttgggtg ttgctaaaga gctgtttatc
      241 aaaaacggtt acaatgctac taccactggt gagattgtga aattgtctga atcttctaaa
      301 gggaatctat actatcattt caaaacgaag gaaaatttgt tcttggaaat cttgaacatt
      361 gaagaaagta aatatcaaga acaatggaaa aaggagcaga ttaaagctaa aactaataga
      421 gaaaagtttt atctgtataa tgagttgtct ttgactacac aatattacta tccactacag
      481 aacgcaataa tcgagtctta cactgaatac tataagacta actccatcaa tgaaaaacag
      541 aacaaatacc aaaataagta tatcgatgca tatcacgtta ttttcaaaga aggtaatctt
      601 aacggtgagt ggagtataaa tgatgttaat gccgtttcta aaattgctgc taacgctgtt
      661 aacggtattg ttacattcac acatgagcaa aacatcaacg aaagaattaa gttgatgaac
      721 aagttctctc agatcttttt gaatggtctt agtaaaggtt ctggtcacca tcatcaccac
      781 cattaatgaa ccaggcatca
//
