LOCUS       sequence-124982-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj12-olsa
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    252..270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             378..389
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             405..1115
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 gaattcgcgg ccgcttctag agacagaaaa gcccgccttt cggcgggctt tgcccagaat
       61 cccttcgagc aaatcctagc gaggtgccgc tagattcgct caaaggaacc cttcttcctg
      121 cccgtctgga atccaggaca cctgccaagg gcctttcggc tggtatgtat ttcgtcctat
      181 ttgggactca tcagctggat gtacgaaggc agcgccgtgc tcagtatctc tatcactgat
      241 agggatgtca atctctatca ctgataggga gctagccaat tgatgcatat aaatgtgagc
      301 ggataacatt gacattgtga gcggataaca agatactgag caccaattct accattcacc
      361 tcttggattt gggtattaaa gaggagaaag gtaccatgac tagtcgtaaa ggcgaagagc
      421 tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt
      481 tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca
      541 tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg
      601 gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg
      661 ccatgccgga aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca
      721 aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag
      781 gcattgactt taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca
      841 gccacaatgt ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa
      901 ttcgccacaa cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc
      961 caatcggtga tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc
     1021 tgtctaaaga tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag
     1081 cgggcatcac gcatggtatg gatgaactgt acaaagctag cgcagcgaac gacgaaaatt
     1141 acgcccttgc agcgtaataa ctgcag
//
