LOCUS       sequence-124984-        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj11
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    265..283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    290..308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             439..450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             466..1176
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    1183..1203
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agcgctgctc tcgtcgatcc cttaagcgcc ggcgaagatc
       61 tcacagaaaa gcccgccttt cggcgggctt tggcctcgca taaatctgtc acagcatcaa
      121 cgtttccgct gaactgtgac agactcacac aatcaaccct ccccggtgga atccaggaca
      181 cctgccaagg gcctttcggc tggtatgtat ttcgtcctat ttgggctcat cagctggatg
      241 tacccgggaa agaagtgctc agtatctcta tcactgatag ggatgtcaat ctctatcact
      301 gatagggata cgtacgatcg ggtgcgagcg gatcgagatg catgctagca taaatgtgag
      361 cggataacat tgacattgtg agcggataac aagatactga gcaccctcgc ataatttcac
      421 ttcttcaatc ctcccgttaa agaggagaaa ttatgaatga ctagccgtaa aggcgaagag
      481 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      541 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      601 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      661 ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc
      721 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
      781 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
      841 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
      901 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
      961 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
     1021 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
     1081 ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca
     1141 gcgggcatca cgcatggtat ggatgaactg tacaaatgat gatactagta gcggccgctg
     1201 cag
//
