LOCUS       sequence-124985-        4579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj11
FEATURES             Location/Qualifiers
     source          1..4579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    265..283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    290..308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             439..450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             466..1176
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    1183..1203
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1239..1270
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(1369..2184)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2092..2111
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2176..2195
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(2542..3492)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3540..3762
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4254..4348)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(4504..4533)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agcgctgctc tcgtcgatcc cttaagcgcc ggcgaagatc
       61 tcacagaaaa gcccgccttt cggcgggctt tggcctcgca taaatctgtc acagcatcaa
      121 cgtttccgct gaactgtgac agactcacac aatcaaccct ccccggtgga atccaggaca
      181 cctgccaagg gcctttcggc tggtatgtat ttcgtcctat ttgggctcat cagctggatg
      241 tacccgggaa agaagtgctc agtatctcta tcactgatag ggatgtcaat ctctatcact
      301 gatagggata cgtacgatcg ggtgcgagcg gatcgagatg catgctagca taaatgtgag
      361 cggataacat tgacattgtg agcggataac aagatactga gcaccctcgc ataatttcac
      421 ttcttcaatc ctcccgttaa agaggagaaa ttatgaatga ctagccgtaa aggcgaagag
      481 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      541 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      601 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      661 ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc
      721 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
      781 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
      841 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
      901 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
      961 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
     1021 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
     1081 ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca
     1141 gcgggcatca cgcatggtat ggatgaactg tacaaatgat gatactagta gcggccgctg
     1201 caggagtcac taagggttag ttagttagat tagcagaaag tcaaaagcct ccgaccggag
     1261 gcttttgact aaaacttccc ttggggttat cattggggct cactcaaagg cggtaatcag
     1321 ataaaaaaaa tccttagctt tcgctaagga tgatttctgc tagtattatt agaaaaactc
     1381 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     1441 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccaaa gaatggcaag
     1501 gtcctggtaa cggtctgcga ttccgacccg tccaacatca atacaaccta ttaatttccc
     1561 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     1621 gaatggcaag agcttgtgca tttctttcca gacttgttca acaggccagc cattacgctc
     1681 gtcatcaaaa tcactcgcat caaccaaacc gttattcatg cgtgattgcg cctgagcaag
     1741 acgaaataca cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gtaaccggcg
     1801 caggaacacg gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     1861 ctggaaggct gttttcccag gaatcgcggt ggtgagtaac cacgcatcat caggagtacg
     1921 gataaaatgc ttgatggtcg ggagaggcat aaactccgtc agccagttga gacggaccat
     1981 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     2041 atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     2101 ccatttatac ccatataaat cagcgtccat gttggagttt aagcgcggac gggagcaaga
     2161 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     2221 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     2281 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agctctagta
     2341 gttacattgt cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc
     2401 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
     2461 gatatctaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     2521 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     2581 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat
     2641 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     2701 tagtgttttt cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt
     2761 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     2821 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     2881 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     2941 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     3001 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     3061 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     3121 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     3181 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     3241 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     3301 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     3361 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     3421 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     3481 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     3541 ggctagtcaa tgataattac atgtcctttt cctttgagtt gtgggtatct gtaaattctg
     3601 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     3661 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     3721 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     3781 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     3841 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     3901 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     3961 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     4021 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     4081 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     4141 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     4201 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca
     4261 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     4321 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagctc
     4381 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     4441 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg
     4501 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt
     4561 agttagccct tagtgactc
//
