LOCUS       sequence-124996-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C Trans V2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-257
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..166)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(279..290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tagcttaaca attaattaac atatcgagat cgaaatcgtc cagagcatcg gagccgagca
       61 tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca aagtcatcga
      121 gggcatcaga acccagcatg tcgagatcga aatcgtccag cgcgtcggcg cgccctccgc
      181 tgcccttctc ggtcatggtg caattatgga cgacaacctg gttggcaagc aaaaactgat
      241 gatcagaatc atcagataaa gtaatcccat aataatcgtc ttccttcaat tcttgtaact
      301 cgaaataaaa tccgcggcac tcacgtgcaa aagcagcggc gggagcaggc ctgaattttt
      361 tagagccggc acacttcgaa agaacgttaa gcaaaacgga tccgcctccg gacccgccac
      421 cagatccacc tccggaccca cctcctgagc ctccacctga tccgccccca gatcccctca
      481 ggtgggtttt caggtggcgt ttgaggctgg cgcccacgga gaagttgcgc atgcagatcc
      541 gacactgaaa tggtttttct ccagtgtgtg ttctaaggtg atttgtaaga tgtggcctct
      601 ggctaaaatt tcgcatacag atgcggcact ggaagggttt ttcgccggtg tgggtgcggg
      661 tatggcggtg cagatgatct ttcttagaaa agtttctcat gcagattctg cactggaaag
      721 gtctctcccc gtccttctgc ctcagatgag tcttaaggtg tctatccaag ttcactgtct
      781 ggctaaagtt gcgcatgcaa attcgacact gaaatggttt ttcgccggta tgagtccgca
      841 ggtggttttc aagcacttct ctgcgagaaa agtttcgcat gc
//
