LOCUS       sequence-124998-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C Trans V3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-259
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..158)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(271..282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caattaatta acatatcgag atcgaaatcg tccagagcat cggagccgag catgtccaga
       61 tcaaagtcat ccaatgcgtc gcttcccaac atatccaggt caaagtcatc gagggcatca
      121 gaacccagca tgtcgagatc gaaatcgtcc agcgcgtcgg cgcgccctcc gctgccccac
      181 agcacgctgt cgcaattatg gacgacaacc tggttggcaa gcaaaaactg atgatcagaa
      241 tcatcagata aagtaatccc ataataatcg tcttccttca attcttgtaa ctcgaaataa
      301 aatccgcggc actcacgtgc aaaagcagcg gcgggagcag gcctgaattt tttagagccg
      361 gcacacttcg aaagaacgtt aagcaaaacg gatccgcctc cggacccgcc accagatcca
      421 cctccggacc cacctcctga gcctccacct gatccgcccc cagatcccct caggtgggtt
      481 ttcaggtggc gtttgaggct ggcgcccacg gagaagttgc gcatgcagat ccgacactga
      541 aatggttttt ctccagtgtg tgttctaagg tgatttgtaa gatgtggcct ctggctaaaa
      601 tttcgcatac agatgcggca ctggaagggt ttttcgccgg tgtgggtgcg ggtatggcgg
      661 tgcagatgat ctttcttaga aaagtttctc atgcagattc tgcactggaa aggtctctcc
      721 ccgtccttct gcctcagatg agtcttaagg tgtctatcca agttcactgt ctggctaaag
      781 ttgcgcatgc aaattcgaca ctgaaatggt ttttcgccgg tatgagtccg
//
