LOCUS       sequence-125-exp        7525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2678
FEATURES             Location/Qualifiers
     source          1..7525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             576..596
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(686..708)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5099..5121)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5221..5240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5318..5336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5404..5508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5509..5577,5578..6369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5727..5746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6540..7128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7029..7048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7282..7299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7416..7437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7452..7457,7458..7475,7476..7482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7490..7506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7495..7517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7514..7525,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7514..7525,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaagagcat
       61 ttgaatcaga atatggagaa cggagcatga gcattttcga agttttttag atgcactaga
      121 acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt ctgcagcctt
      181 acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg tgggtgcaaa
      241 gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt ttccatgtac
      301 cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa aaaggtggaa
      361 aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat tgtgaaatta
      421 gaaatcttca aactataatc atctcactgg atccccggga ttggccaaag gacccaaagg
      481 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggctagagg
      541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
      601 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg
      661 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      781 aggcccgcac cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact
      841 aactaacttg ttctgacata attttcagct tgaatggcga atggcgcttt gcctggtttc
      901 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
      961 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga
     1021 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     1081 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     1141 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     1201 taagtttaat taagttgata ctaactaaca agatctgatt aattttcagg acagtcgttt
     1261 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
     1321 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
     1381 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
     1441 tgccactcgc tttaatgatg atttctcccg cgctgtactg gaggctgaag tccaggtaag
     1501 tttaaacagg atcttactaa ctaacatgct aacactgaat tttcagatgt gcggcgagtt
     1561 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
     1621 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
     1681 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     1741 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     1801 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     1861 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     1921 gacgatggtg caggatgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa
     1981 ttttcagatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     2041 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     2101 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     2161 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     2221 gatcatctgg tcgctgggga atggtaagtt taaacagttg aatactaact aacggagatc
     2281 tttgaaattt tcagaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
     2341 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
     2401 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
     2461 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgattctttg
     2521 cgaggtaagt ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagtacgc
     2581 tcacgcgatg ggcaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
     2641 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     2701 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
     2761 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgtaagttta
     2821 aacaataacc tactaactaa cgtagataat ttaaattttc aggctgacgg aagcaaaaca
     2881 ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata
     2941 cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc
     3001 gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact
     3061 gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca
     3121 accgaacgcg accgcatggt cagaagccgg gcacatcagc gcatggcagc agtggaggta
     3181 agtttaaaca agatcctact aactaactct acattgatga attttcagac tggcggaaaa
     3241 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
     3301 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
     3361 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
     3421 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
     3481 ctgggtcggt aagtttaaac aaagttgtac taactaacga agatcttgat aattttcaga
     3541 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga
     3601 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac
     3661 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt
     3721 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct
     3781 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga
     3841 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtagtaagt
     3901 ttaaacttga tagtactaac taacatgttt catttaaatt ttcagtaccc cgtacgtctt
     3961 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
     4021 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
     4081 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
     4141 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc aactgagcgc
     4201 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga
     4261 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg
     4321 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     4381 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     4441 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     4501 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     4561 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     4621 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     4681 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     4741 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     4801 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     4861 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     4921 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     4981 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     5041 ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     5101 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5161 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     5221 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5281 gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta
     5341 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5401 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5461 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5521 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5581 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5641 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5701 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5761 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5821 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5881 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5941 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6001 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6061 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6901 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     6961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7261 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7501 taacaatttc acacaggaaa cagct
//
