LOCUS       sequence-125000-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty Target minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-282
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(203..222)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    268..323
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(322..341)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(684..700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(684..700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(697..719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    708..724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tgattaattc gagcgaacgc gtagccaaag gcaacaggcg
      121 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
      181 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
      241 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
      301 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
      361 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
      421 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
      481 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
      541 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
      601 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
      661 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      721 acaattccac acaacatac
//
