LOCUS       sequence-125013-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1x Target 1 0gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-289
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    162..183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(198..203,204..221,222..228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    236..252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     241..263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     260..276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     260..276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     619..638
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    637..692
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccggacc acttcccctc cagcacctcc tgcagcttct tcaagatccg ctggcacgac
      121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      181 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      241 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg
      301 caggtcgagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
      361 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa
      421 taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa
      481 ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt
      541 catggcagcc agcatatggg catatgttgc caaactctaa accaaatact cattctgatg
      601 ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca
      661 cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt
      721 catgatgtcc ccataatttt tggcagaggg aa
//
