LOCUS       sequence-125020-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3x Target 1 0gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-299
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    250..271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(286..291,292..309,310..316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    324..340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     329..351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     348..364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     348..364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     707..726
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    725..780
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccggacc acttcccctc cagcacctcc tgcagcttct tcaagatccg gaccacttcc
      121 cctccagcac ctcctgcagc ttcttcaaga tccggaccac ttcccctcca gcacctcctg
      181 cagcttcttc aagatccgct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      361 tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga
      421 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
      481 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      541 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      601 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      661 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
      721 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
      781 a
//
