LOCUS       sequence-125034-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express recepter TVA for envA with AAV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Menegas W, Bergan JF, Ogawa SK, Isogai Y, Umadevi Venkataraju K, 
            Osten P, Uchida N, Watabe-Uchida M
  TITLE     Dopamine neurons projecting to the posterior striatum form an 
            anatomically distinct subclass.
  JOURNAL   Elife. 2015 Aug 31;4. doi: 10.7554/eLife.10032.
  PUBMED    26322384
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-31"; volume: "4. doi"; pages: " 10.7554/eLife.10032"
COMMENT     Sequence Label: pAAV-EF1a-flex-TVA
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(118..151)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(171..194)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    651..684
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    735..768
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(846..866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tgaggtaccg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
       61 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
      121 acttcgtata gcatacatta tacgaagtta tggcgcgcta gtggatccta cttgtcgtcg
      181 tcgtccttgt agtccttgga tgcgctttca agactgaaga tgtcagacct gctttttgct
      241 ttggacttcc cccatgcagc gataccaccc acagctacca ggcagcacag gagcactgca
      301 gtgatcagca tccacatgcg gccgtgattc ctggctggca gagcacgtcc aggagcaggg
      361 gcagtgggag cctctgtgcc gttgtccgtg ggcaccgcgg ggatcgcgct ggtcccgcag
      421 ccccactcgt cccgcccgtc gtcgcagtcg gggtgtccgt cgcacagcca gtcctgcggg
      481 taacactccc cgtgggcacc gggcggctcc gagcagcgga actgaccggg ggggcaacgg
      541 gacaaagaac cgttaccgga cccgttaccg gtcacgttac cgggcagcag cagcagcagc
      601 agcgcgggca gcagccgcgc catgtcggta ccaccacatc aagctctagc ataacttcgt
      661 ataaagtatc ctatacgaag ttatttgcct taacccagaa attatcactg ttattcttta
      721 gaatggtgca aagaataact tcgtataatg tatgctatac gaagttatga attcgatatc
      781 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
      841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
      901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
      961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1081 ttccccctcc ctattgccac ggcggaa
//
