LOCUS       sequence-125040-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Packaging plasmid for producing MuLV retroviral particles. Use in 
            conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pUMVC
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          145..414
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             468..884
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     814..836
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     852..868
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      121 cggattcccc gtgccaagag tgacgtaagt accgcctata gactctatag gcacacccct
      181 ttggctctta tgcatgctat actgtttttg gcttggggcc tatacacccc cgcttcctta
      241 tgctataggt gatggtatag cttagcctat aggtgtgggt tattgaccat tattgaccac
      301 tccaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc gcgccaccag
      361 acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct gcagtcaccg
      421 tcgtcgacgg tatcgataag cttgatatcg aattcgccgc caccatgggc cagactgtta
      481 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
      541 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
      601 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
      661 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
      721 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
      781 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
      841 ctcgatcctc cctttatcca gccctcactc cttctctagg cgccaaacct aaacctcaag
      901 ttctttctga cagtgggggg ccgctcatcg acctacttac agaagacccc ccgccttata
      961 gggacccaag accaccccct tccgacaggg acggaaatgg tggagaagcg acccctgcgg
     1021 gagaggcacc ggacccctcc ccaatggcat ctcgcctac
//
