LOCUS       sequence-125044-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr 
            driving expression of yEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     pRS416-yZ3EV-Z3pr
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(725..743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(753..770)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(753..769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(762..784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
       61 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
      121 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
      181 ctttaactta tggtgttcaa tgtttttcta gatacccaga tcatatgaaa caacatgact
      241 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
      301 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
      361 tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
      421 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag
      481 ttaacttcaa aattagacac aacattgaag atggttctgt tcaattagct gaccattatc
      541 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca
      601 ctcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat
      661 ttgttactgc tgctggtatt acccatggta ttgatgaatt gtacaaataa ggtacccaat
      721 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
      781 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      841 tggcgtaata gcgaagaggc ccgcaccgat cgccc
//
