LOCUS       sequence-125046-       12668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as 
            gene of interest for integration on cxtTi10816, Mos Chr IV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR04
FEATURES             Location/Qualifiers
     source          1..12668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2999..3023
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3030..3046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3030..3046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3979..4012)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4041..4061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        5356..5955
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     terminator      complement(6772..7095)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(7109..7142)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7171..7191
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7204..7506,7560..7634,7686..7805,7857..8072)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8304..8321)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8304..8320)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10261..10272
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10418..10442)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11877..11897)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(12101..12120)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12220..12242
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12280..12298)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12366..12470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12471..12539,12540..12668,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgc ggtacccaag gcgacaacgt aagtgactga aggcattaaa
     1621 ggtacatgcg tccgcgcaag tgtccgcttt tgctggccta acaagtaaga gaaacaagta
     1681 agaaagagta agagaaaact cacccgaggt gtctcttcta gcaaccgcct attcagataa
     1741 ttttaagcca gttcgagagt tatttgtcgc atggtataga agctcgttat ttacattaaa
     1801 tttttagtct tcccgcagaa gtggacgatg cgatgggcta cttcaaaaat ctgacgccac
     1861 gtgtcaagga ccttcacaag agcatgacaa acttggaaga tattagcaag tggcagaaga
     1921 aggccagttt ctctggcacc cttgagaatt actcgcgtct cggtgacaag atcaatgtga
     1981 aaccatttat ggatgctgtt gatgctagaa tgggtgccga agctgatgcc gtgaaagggg
     2041 tataaagcta ctgttgcacg acgactcaca gactaatcat tccaggtcct cgcgatttgt
     2101 gaaaaataca agtcattcta ccaaaacgag ggaaaacttc acgcggacag tatcgccaat
     2161 ttgaatagga ctcggctcga catggacagt gcggcggata aatatgcgaa caacgagact
     2221 gaagttaaca agactcgttt ggataacagt accacggaat ttgaagtggc ttgtgagagg
     2281 tttgtaggtc tttcgtaggc acgatatcct tatttgaatg tgtttcagaa tgcgagaact
     2341 ggcgaacgga atcaagacaa ttgaatcgaa ccattcttcc tggcaagacg gtcttatgaa
     2401 ggagattaaa gtggcgttgc gtaaataaag aaaggatcct ctttgaaaat aaagattttc
     2461 tacattcact tattttttta gctttatact tttcagatcg aatgaactgt ttccaacgtt
     2521 tccatattaa ttttttttag ttaaaaaagt tgggtattaa cagttttgtt aacagttttg
     2581 tcttccgaca gatgtgagac ggaaaaatgg ccaaaattcg agattttgac taaaatcagt
     2641 gtagcttttt caaaattttg aaccggcatg aaaaagtttg aaaagttttc gagaagtttc
     2701 aatccgttca tttgagccca tgttgggtct ataagatcaa aatcgcccga actgttagtc
     2761 ctcctttaag ttacgattat taacaaaatg tcgcctaagt ttagtttcac acttgtctca
     2821 cactagtcga tgaactgtgt gactactgta gtgagtacag tagggcccgt gtaaaaatac
     2881 aaaaaccaag aaaaagtaaa ccaaaataaa aaacgaattt attgttcact acaaccaaac
     2941 tgcaaagttc ttttcttacc catcagctca atcgtgtact tgcgtggaaa ccctaggagc
     3001 cactttgtac aagaaagctg ggtcacgggc aggaaacagc tatgaccatg attacgccaa
     3061 gctacgtaat acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc
     3121 tcgaggaatt cctgcagttc aatcggtttt tccttgcttg cgcccaattc tgatggttct
     3181 tccatttcta aattttagtt taataaaaaa attttaaaat aatacgagtt ttgagactaa
     3241 ctttcaataa aaaccaaaaa aaattgcttc aaaaaagagt tcgcgccgat ttacgataaa
     3301 cacagcgcgc tacgacactc cgaaaaccgc gccgcaccat tacattctcc gccccaccgc
     3361 acggtttgcg gaaggcgatg aaattgctgg gcctaaaatt ttaacccttt ttttcccgtt
     3421 tttcaactgt tttcccgatt ttttcgatat ttttttcagg tttttagtta gaaaagtata
     3481 taaaaattgc attatttgta gttttcgctc gtaatttgtg atagaaaacg gccaatttgc
     3541 ctagatttgt gattctacca agtggaattt ttgggaaaat atcaatattc cacgattttt
     3601 attcaaatca taacgtttcg aaattatcag cttaaataaa actaaaataa ataataaaat
     3661 actaaaaacc ctgaatttct cggttctcgg ttcagcttga gttcgttcta tacacgttat
     3721 cgatttttgt catttcgttt aatcatgggt tttgattcat tttaaatatg aaatttgatt
     3781 tattcctcgt ttcgaagttg gtttgtcttg tccggccggt aaacaaatgt tattaaaaaa
     3841 ataataaatt tcgaaatttt tcgtaatgtt ccgcaatcgt gtttttccgt ttatctcgtc
     3901 ttcccttctt tcaaatatac tatttatttt ctcaattcac atattttcag ccagccccgc
     3961 tcaacaagtg gaataaaaat aacttcgtat agcatacatt atacgaagtt attctagagg
     4021 atccagaaaa aatgactgct ccaaagaaga agcgtaaggt accggtagaa aaaatgtcca
     4081 agggagagga ggacaacatg gccatcatca aggagttcat gcgtttcaag gtccacatgg
     4141 agggatcagt caacggacac gagttcgaga tcgagggaga gggagaggga cgtccatacg
     4201 agggaaccca aaccgccaag ctcaaggtaa gtttaaacat atatatacta actaaccctg
     4261 attatttaaa ttttcaggtc accaagggag gaccactccc attcgcctgg gacatcctct
     4321 ccccacaatt catgtacgga tcaaaggcct acgtcaagca cccagccgac atcccagact
     4381 acctcaagct ctccttccca gagggattca agtgggagcg tgtcatgaac ttcgaggacg
     4441 gaggagtcgt caccgtcacc caagactcct ccctccaaga cggagagttc atctacaagg
     4501 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gtcaagctcc
     4561 gtggaaccaa cttcccatcc gacggaccag tcatgcaaaa gaagaccatg ggatgggagg
     4621 cctcctccga gcgtatgtac ccagaggacg gagccctcaa gggagagatc aagcaacgtc
     4681 tcaagctcaa ggacggagga cactacgacg ccgaggtcaa gaccacctac aaggccaaga
     4741 agccagtcca actcccaggt aagtttaaac atgattttac taactaacta atctgattta
     4801 aattttcagg agcctacaac gtcaacatca agctcgacat cacctcccac aacgaggact
     4861 acaccatcgt cgagcaatac gagcgtgccg agggacgtca ctccaccgga ggaatggacg
     4921 agctctacaa gtaagaattc gctagccggc catacaagta atccggatga tcgacgccaa
     4981 cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt
     5041 attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat
     5101 ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg
     5161 caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg
     5221 ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca
     5281 attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa
     5341 aactagttct agagcgcacc tttggtcttt tattgtcaac ttccattggt tcttccattg
     5401 tttctgttaa attaatgaat ttttcataaa ataaagacat tatacaatat aaaaatgaag
     5461 aatttattga aaataaactg ccagagagaa aaagtatgca acactcccgc cgagagtgtt
     5521 tgaaatggtg tacggtacat tttcgtgcta ggagttagat gtgcaggcag caacgagagg
     5581 gggagagatt tttttgggcc ttgtgaaatt aacgtgagtt ttctggtcat ctgactaatc
     5641 atgttggttt tttgttggtt tattttgttt ttatctttgt ttttatccag attaggaaat
     5701 ttaaatttta tgaatttata atgaggtcaa acattcagtc ccagcgtttt tcctgttctc
     5761 actgtttagt cgaattttta ttttaggctt tcaacaaatg ttctaactgt cttatttgtg
     5821 acctcacttt ttatattttt ttaattttta aaaatattag aagtttctag gataattttt
     5881 tcgactttta ttctctctac cgtccgcact cttcttactt ttaaattaaa ttgttttttt
     5941 ttcagttggg aaacactttg ctcaatgtcc gaactcatca aggagaacat gcacatgaag
     6001 ctctacatgg agggaaccgt agacaaccac cacttcaagt gcacctccga gggagaggga
     6061 aagccatacg agggaaccca aaccatgcgt atcaaggtcg tcgagggagg accactccca
     6121 ttcgccttcg acatcctcgc cacctccttc ctctacgggt ccaaggtaag tttaaacata
     6181 tatatactaa ctaaccctga ttatttaaat tttcagacct tcatcaacca cacccaagga
     6241 atcccagact tcttcaagca atccttccca gagggattca cctgggagcg tgtcaccacc
     6301 tacgaggacg gaggagtcct caccgccacc caagacacct ccctccaaga cggatgcctc
     6361 atctacaacg tcaagatccg tggagtcaac ttcacctcca acggaccagt catgcaaaag
     6421 aagaccctcg gatgggagac cttcaccgag accctctacc cagccgacgg aggactagag
     6481 ggacgtaacg acatggccct caagctcgtc ggaggctccc acctcatcgc caacatcaag
     6541 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gaccacctac
     6601 cgttccaaga agccagccaa gaacctcaag atgccaggag tctactacgt tgactaccgt
     6661 ctagagcgta tcaaggaggc caacaacgag acctacgtcg agcaacacga ggtcgccgtc
     6721 gcccgttact gcgacctccc atccaagctc ggacacaagc tcaactaatg agactttttt
     6781 cttggcggca caataaagtt tcctcttttt gttgggtcac tcaacgaaca caatacaaat
     6841 atttgagcgc tcgcattttt ctcaaccttt cgggtttaaa gcacttcaaa atggaattga
     6901 agagagattg tgaaagtaca tcagacagaa tcacattttg atagcattca cttcactcag
     6961 atgcaaactt ttcagtgact ataaaaagtc ataaaataat aacattaaaa aagcaaataa
     7021 attagcgaga gaattttttg acaaaaagaa agaagagtga tagagaagaa gggaatgctt
     7081 gaaaggattt tgcatttatc gcggccgcat aacttcgtat agcatacatt atacgaagtt
     7141 attctagagg atccagaaaa aatgactgct ccaaagaaga agcgtaaggt accggtagaa
     7201 aaaatgtcca agggagagga gctcttcacc ggagtcgtcc caatcctcgt cgagctcgac
     7261 ggagacgtca acggacacaa gttctccgtc tcaggagagg gagagggaga cgccacctac
     7321 ggaaagctca ccctcaagtt catctgcacc accggaaagc tcccagtccc atggccaacc
     7381 ctcgtcacca ccttcactta cggagtccaa tgcttctccc gttacccaga ccacatgaag
     7441 cgtcacgact tcttcaagtc cgccatgcca gagggatacg tccaagagcg taccatcttc
     7501 ttcaaggtaa gtttaaacat taattaatac taactaaccc tgattattta aattttcagg
     7561 acgacggaaa ctacaagacc cgtgccgagg tcaagttcga gggagacacc ctcgtcaacc
     7621 gtatcgagct caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     7681 ttcagggaat cgacttcaag gaggacggaa acatcctcgg acacaagctc gaatacaact
     7741 acaactccca caacgtctac atcatggccg acaagcaaaa gaacggaatc aaggtcaact
     7801 tcaaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagatcc
     7861 gtcacaacat cgaggacgga tctgtccaac tcgccgacca ctaccaacaa aacaccccaa
     7921 tcggagacgg accagtcctc ctcccagaca accactacct ctccacccaa tccgccctct
     7981 ccaaggaccc aaacgagaag cgtgaccaca tggtcctcaa ggagttcgtc accgctgccg
     8041 gaatcaccca cggaatggac gagctctaca agtaggaatt cgctagccgg ccatacaagt
     8101 aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     8161 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     8221 attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgcag
     8281 gccttaagtg agtcgtatta cggactggcc gtcgttttac acccgtgcag ttgaaattga
     8341 aaatgagtta aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag
     8401 cgagtgtgac aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa
     8461 tgcattgaac atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt
     8521 aatcacattt ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt
     8581 aaatatttat gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac
     8641 gaaatagaag ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa
     8701 agagattagt cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag
     8761 ttgagggaac tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa
     8821 ttcaaagtca aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa
     8881 atgatggatt gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt
     8941 caaaaagttt ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc
     9001 attctcctga tcgttctccg gtttggcgat ctcgaaaagc acttgctcag tgtccagatc
     9061 acggatttgg aacttggtga actcgatgtt atagatgttc gcagatgggg agcataagaa
     9121 tcctaaattt atgttttaaa ctgaaatcca aagggagcaa gataccttga gtgattcccg
     9181 gaagtgctaa aacgtcgttc ggagtgattt gagctttctt cgcaagctcc gattccgttg
     9241 tgattccttg ttcggtgctt ggtggtggcc gtggcatctg gaaatatgga aaagttcaac
     9301 aaaaagaaaa gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag
     9361 ttttctgagt ctcacctgcg acgggaaggt cgccgagccg ggtggaatcg atcgttgttg
     9421 ctcggctttc atatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga
     9481 aaagagtgtg gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt
     9541 tgtttttttt tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc
     9601 cggaaaactg gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg
     9661 taaattatca tttttattga aactccacca tttttctatt tataacgcta ataatttgaa
     9721 aaagaaacct attgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg
     9781 attgattcga atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc
     9841 gagcagcaca caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc
     9901 ttcgaatgat tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga
     9961 aaagaagcag aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag
    10021 tcatagaagt agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga
    10081 gtatgcaaag gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa
    10141 gaagagttga aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg
    10201 atgaattgaa atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta
    10261 atagaagggc gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa
    10321 gtggtcaact gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt
    10381 cgaatacttt ttacaaggac ttggataaat tggctcaagc ctgctttttt gtacaaactt
    10441 gcactagtga cacacggcca aatttcttat tttcctgcta ttttctccaa atatgattaa
    10501 aatttgttca tttcaaaatt gcctccaact tgttttgggg caaaaatttc acaaaacttc
    10561 acagcaaaat gtgaaacaat attgctatct attgaaaaca ttaatgatag tcgagtgact
    10621 tcttgaacca atactcatcg catcaaatgc cttttctcga atactctgta ataattggca
    10681 tataaaaaca tacatactca tcagtttgaa agctaacctt taccataatc attactaaaa
    10741 ttggatttaa agccggacac caagaaaaaa gatacggtag ataaactgac agatgagaag
    10801 tctcgatacg aaataatggc attagaaaag acacatagaa tggaatgtgg atagtgagaa
    10861 atggtagaat gaactgaaaa attgagaatt taaggagaaa tgttattttt aagctgcttt
    10921 gcttgaaact ttgaatttaa aattttagaa caattactgt ttcaaaatat ttttattttt
    10981 atttactcca tttgaaaaaa aagcaacatt gcttgttcaa tttctgttat tgccttacct
    11041 gaaccataag taccatattc aattgttgtt gagacttcat cgaatttgta ctccgacttc
    11101 ccttatgaat ttcgtatctg ataattgtcc aacaaaccat cgcaattact gtagttatca
    11161 ctagaacagc cattatataa atcgtacatg cccatgaaat tgggtcagca acaaagtcat
    11221 tcaacgtgta atcatatctt tctagtgatg caattaaata tgcagatttt ccgattccat
    11281 tcagattgta tacgatatat tctgatagtt gatcgtcagc agcatctggg acatagccaa
    11341 aggtgattag aactgtggat attagagaga caataaatga tatgattgtg gttgtaagga
    11401 ccacgttttt ttgtgttctc gttgttctga aagtataatt tgtagattct taaactcaga
    11461 atttatcata tttgaactcc gctgtttttg aattaagtaa tagctgaaac agtgaacatt
    11521 agtttaaaaa tataatttta caggcacgca caaacttcgg tgtgggtacg tattacctgc
    11581 ctcctattga atattcattg tttaaacgtt ttcagaaaaa aagtgtacat gttttttgga
    11641 gtatgatgat gatgtagtcg caaggcagat atgaaatgta ggtaacacag tgcacagatg
    11701 cgttttttgc caacatcaag ggttgtaagt aacttttcag agaaaaacat atagaaattt
    11761 tgtttcaagt atcgtatcag tttttttctg attaacttat aaaaagaact cttcgacaat
    11821 ttgaatgctt aatttaaaaa aattaagtcg aatatttcag catgccaacc cgggagcaac
    11881 ttttctatac aaagttgata gcttggcgga tccctggcgt tacccaactt aatcgccttg
    11941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    12001 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
    12061 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
    12121 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    12181 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    12241 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
    12301 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    12361 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    12421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    12481 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    12541 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    12601 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    12661 ttccaatg
//
