LOCUS       sequence-125048-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos 
            Chr II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR06
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..111)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    140..173
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 cttgtgtccg ttgacgtctc cgtcgagctc gacgaggatt gggacgactc cggtgaagag
       61 ctcctctccc ttggacattt tttctaccgg taccttacgc ttcttctttg gagcagtcat
      121 tttttctgga tcctctagaa taacttcgta taaggtatac tatacgaagt tatgcggccg
      181 ctctagaact agttttcctt cctcctctat atatttttat gttttctttt aaaccttcct
      241 ccatatttct gaattgaatg ttgttgcata ttgtgtgtcc ttatttcatt tccatcatcc
      301 ttttgtgtgt ttcccccttc caccaattcc ccccacgtcc taatgaaatg aaagcgagaa
      361 gataaataaa cttgcacatt gtgttcagat gagaggagcg gaatacgaat tgggagacgg
      421 aaagagaaaa aaaaatgttt agatttggat tgaattaatt ttttagaaag caagaaaaaa
      481 aacacaaaga gaataaataa ataataggaa aaaaaacaaa tattcagtat ttaaaatttg
      541 aaaattcaac gacgttggcg tcgatcatcc ggattacttg tatggccggc tagcgaattc
      601 ttacttgtag agctcgtcca ttcctccggt ggagtgacgt ccctcggcac gctcgtattg
      661 ctcgacgatg gtgtagtcct cgttgtggga ggtgatgtcg agcttgatgt tgacgttgta
      721 ggctcctgaa aatttaaatc agattagtta gttagtaaaa tcatgtttaa
//
