LOCUS       sequence-125059-         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene 
            (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr 
            II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR13
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..100)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    129..162
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 tgacgtctcc gtcgagctcg acgaggattg ggacgactcc ggtgaagagc tcctctccct
       61 tggacatttt ttctaccggt accttacgct tcttctttgg agcagtcatt ttttctggat
      121 cctctagaat aacttcgtat aaggtatact atacgaagtt atgcggccgc ccattatcat
      181 cattgtgacg cttttcattg aacaacaaaa caagacattt ttcaaattaa ttatcgtttt
      241 ttccgttgta ttttcaactt ttttgatgtg gttaaatacc cccctcaatg ctctattttt
      301 gctctttttc agctcgaaaa ctggaatttc tactgaaatt tacgtaattt ttgccatttt
      361 ttcgctgaaa tttgntagaa atcgctaaaa aatgcgaatt tttcggaaaa aaagcccgat
      421 ttcctcaatt tt
//
