LOCUS       sequence-125061-        1141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacteroides coprophilus Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1141)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX388 Bacteroides coprophilus Cas9
FEATURES             Location/Qualifiers
     source          1..1141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccatg aagaaaacac tgggactgga cctggggact aacagcattg ggtgggccgt
      361 catcaactca aacatcgact cagaaggaaa agaaaagctg gtggggatca gctcctgcgg
      421 aagccggatc attcctatgg acgctaccac actgggagat ttcggaaagg gcaacactaa
      481 aagtccagtg gcagagcgaa cccgactgcg gggcattcgg agactgctgg aaaggtcact
      541 gctgaggcgc gagcgactgc accgagtgct gtcagtcatg gggttcctgc cagagcatta
      601 cgctagccag ctggaccgct atggaaagtt tctgccagaa accgagccca agctggcatg
      661 gtacaaagac gatagcggca ggtatcagtt cctgtttcag aagtccttcc acgagatgct
      721 ggaggacttt cgacagcatc agccagaact ggtggcagga gagaagaaaa tcccttacga
      781 ttggacaatc tactatctgc ggaagaaagc actgtctcag gagatcacta aggaggaact
      841 ggcctggatt ctgctgaatt tcaaccagaa acgcggctac tatcagctgc gaggggagga
      901 agagcaggaa gagaacaata agagcgtgga gtatcacgcc ctgaaagtgg tgagcgtcga
      961 ggactctgga gaaagaaagg gcaaagatat ctggtacaat gtgactctgg agaacggatg
     1021 ggtctatcga cgggctagca acattcccct ggactggacc ggcaaggtga aagagttcgt
     1081 ggtcactacc gagctggacg atgccgggaa cccaaagaaa gataaggaag gaaatgtgaa
     1141 a
//
