LOCUS       sequence-125063-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Corynebacter diphtheriae Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX377 Corynebacter diphtheriae Cas9
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccatg aagtaccatg tcggaatcga tgtcggaacc ttttctgtgg ggctggctgc
      361 tattgaagtg gatgacgctg gaatgcctat taagaccctg agtctggtgt cacacattca
      421 tgactcagga ctggatcctg acgagatcaa gagcgctgtg accaggctgg caagctccgg
      481 aatcgcccgg agaacaaggc gcctgtaccg acggaagaga aggcgcctgc agcagctgga
      541 taagttcatc cagaggcagg gctggccagt gatcgagctg gaagattaca gcgaccccct
      601 gtatccttgg aaggtgcgcg ccgaactggc cgcttcttat attgctgacg agaaggaacg
      661 gggggagaaa ctgagtgtgg ctctgagaca catcgcaagg catcgcggat ggaggaaccc
      721 ttacgccaag gtgtctagtc tgtatctgcc agatggcccc tcagacgcct tcaaggctat
      781 tagggaggaa atcaaacgcg ctagcggcca gcctgtgcca gagactgcaa ccgtcgggca
      841 gatggtgacc ctgtgcgaac tgggcacact gaagctgcga ggagagggag gagtgctgag
      901 tgcacggctg cagcagtcag attacgcccg cgagatccag gaaatttgtc gaatgcagga
      961 gatcggccag gaactgtatc gcaagatcat tgacgtggtg ttcgcagccg agtccccaaa
     1021 gggctctgcc tcaagccggg tggggaaaga tcctctgcag ccaggaaaga acagagcact
     1081 gaaagccagc gacgcttttc agc
//
