LOCUS       sequence-12507-e       12467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Pierce AJ, Hu P, Han M, Ellis N, Jasin M
  TITLE     Ku DNA end-binding protein modulates homologous repair of 
            double-strand breaks in mammalian cells.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3237-42.
  PUBMED    11751629
REFERENCE   2  (bases 1 to 12467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3237-42."
COMMENT     Sequence Label: pHPRT-DRGFP
FEATURES             Location/Qualifiers
     source          1..12467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2223..2239
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        3565..3944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3947..4222
                     /label=chicken beta-actin promoter
     intron          4223..5231
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5155..5177
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5239..5258
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5316..5336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5358..5378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5592..5601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5598..5600,5601..5603,5604..6317)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSG*G*QGNTYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6251..6272
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6440..6561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6477..6496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6531..6550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(7222..7821)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7305..7325)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7802..7821
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7840..8337)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7888..7907)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8279..8302
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(8425..8444)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8490..8545
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8544..8563)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             8907..8927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8949..8969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      9183..9192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9189..9191,9192..9194,9195..9653)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(9710..9726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9734..9750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9758..9764,9765..9782,9783..9788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9803..9824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      9929..10064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11823..11842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11824..11842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11849..11866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11849..11865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11858..11880)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12007..12462
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12094..12113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12304..12325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtggatcc gcggccgcta attcgattct tctgtaaaat tatcttatta
     2281 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg
     2341 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg
     2401 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta
     2461 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc
     2521 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt
     2581 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc
     2641 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt
     2701 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa
     2761 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag
     2821 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt
     2881 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat
     2941 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta
     3001 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg
     3061 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg
     3121 acagccagag ctatacagag aaatcctgtc tcaaaaaacc aaaaataaag tgtatatact
     3181 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt
     3241 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact
     3301 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg
     3361 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt
     3421 attatctaaa cgtaattaaa tgttgatatt attgaagcat ttatcagggt tattgtctca
     3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3541 ttccccgaaa agtgccacct ggtcgacatt gattattgac tagttattaa tagtaatcaa
     3601 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3661 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3721 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3781 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     3841 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     3901 ctacttggca gtacatctac gtattagtca tcgctattac catgggtcga ggtgagcccc
     3961 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     4021 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg
     4081 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     4141 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4201 aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg
     4261 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     4321 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc
     4381 ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga
     4441 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct
     4501 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc
     4561 gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag
     4621 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc
     4681 tgtaaccccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     4741 gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     4801 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     4861 ggccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4921 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg
     4981 gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag
     5041 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag
     5101 cctcggggct gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     5161 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     5221 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     5281 aattcagatc cgccgccact atgggatcaa gatcgccaaa aaagaagaga aaggtgccga
     5341 agaagcatgc agcaccacca aaaaaaaaac gaaaagtaga agacccacga ggcaacacca
     5401 gcggcgtgct gagcaccccc aaggccaaga gggccaagca cccccccggc accgagaagc
     5461 ccaggagcag gagccagagc gagcagcccg ccacctgccc catctgctac gccgtgatca
     5521 ggcagagcag gaacctgagg aggcacctgg agctgaggca cttcgccaag cccggcgtgg
     5581 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5641 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggctagggat
     5701 aacagggtaa tacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     5761 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     5821 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5881 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5941 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6001 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6061 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     6121 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6181 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     6241 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6301 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     6361 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     6421 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6541 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatctgaag cttctgatgg
     6601 aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga ggctgctgat
     6661 ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag aaccacaaag
     6721 tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata gatcatgagt
     6781 gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat atccaactat
     6841 gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca tttatctaat
     6901 cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag gtactctgtt
     6961 ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac atctttattg
     7021 tttaatagat catcaatttc gatccgctcc tgggcaccga actgcgccgc gtgttcagca
     7081 gggtcggcgt gttcggtgtg tcccccgcgg tgggcctcgg gggcgggtgc ggggtcggcg
     7141 gggccgcccc gggtggcttc ggtcggagcc atggggtcgt gcgctccttt cggtcgggcg
     7201 ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     7261 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     7321 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     7381 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     7441 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     7501 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     7561 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     7621 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     7681 ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc
     7741 gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac
     7801 cgtgggcttg tactcggtca tggtggcggc tggatcggtc gaaaggcccg gagatgagga
     7861 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
     7921 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
     7981 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
     8041 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
     8101 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
     8161 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg
     8221 cctaccggtg gatgtggaat gtgtgaggcc agaggccact tgtgtagcgc caagtgccca
     8281 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
     8341 agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     8401 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     8461 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     8521 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     8581 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg
     8641 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     8701 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     8761 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     8821 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc
     8881 agcccaagct ttagggatca agatcgccaa aaaagaagag aaaggtgccg aagaagcatg
     8941 cagcaccacc aaaaaaaaaa cgaaaagtag aagacccacg aggcaacacc agcggcgtgc
     9001 tgagcacccc caaggccaag agggccaagc acccccccgg caccgagaag cccaggagca
     9061 ggagccagag cgagcagccc gccacctgcc ccatctgcta cgccgtgatc aggcagagca
     9121 ggaacctgag gaggcacctg gagctgaggc acttcgccaa gcccggcgtg gatccaccgg
     9181 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     9241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     9301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     9361 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     9421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     9481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     9661 agaagaacgg catcaaggtg aacttcaata aaagcttggc gtaatcatgg tcatagctgt
     9721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9841 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     9901 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9961 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10021 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagagc ttgtttttat
    10081 ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc tcccctcccc
    10141 tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt actctgtagt
    10201 ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc ttgttctgaa
    10261 aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt aatctcagca
    10321 caggcaagta gagacaagga ttatgattac agtccagcct gggctattta gagggattct
    10381 ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat agtctccact
    10441 tctgcaaaat attgctttat gaagtaagaa ttcccttcat agagacaagg aatgtgtcct
    10501 gtaaaagttt aatgtgtaag aagtatttgt tataaaagat aaatattcag aatcttcttt
    10561 ttaattcctg attttatttc tataggactg aaagacttgc tcgagatgtc atgaaggaga
    10621 tgggaggcca tcacattgtg gccctctgtg tgctcaaggg gggctataag ttctttgctg
    10681 acctgctgga ttacattaaa gcactgaata gaaatagtga tagatccatt cctatgactg
    10741 tagattttat cagactgaag agctactgtg taagtataat taacttataa ttaaaaaaat
    10801 agggccattc tagttttatc tatatttttt ttaaacttgt gcaaactatg ctacataatc
    10861 aacttacttg attatgtaat atatatagta atagttggaa ttactattat tacatattta
    10921 tttttctgtg ctggggtaga acccagggaa ctaagtactg taataaccga gcttcattca
    10981 tcctagtcat ttttaatttt tttttttttt tttgtttttt cgagacaagg tttctctgtg
    11041 tagccctggc tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagaaatct
    11101 gcctgcctct gcctcccagt gctgagatta atgacgtgca ccaccactgc ctggcatttt
    11161 tttaaagttt taatttgaga ccctttctca ctaacttgct cacactatcc tcgaactcat
    11221 tacgtgggct ggtctagaaa tttcagtcaa cttgcctcag cttctcaagt agctgggatt
    11281 acaggcaggt gctaccactt ctggccatga tcattgttgt tgttgctatt attattatta
    11341 ttatttcact gatacttcat atcacaacac agaatagtaa ctgtagttca tgaccaacaa
    11401 aaaagttgta cagtttctct gttgtcttat tttgatctgg attttgattt gtttcatggc
    11461 actgggcagg ctaatctcaa actgcatggc tcaaatagtc cttctgtctt aatctcccat
    11521 gttctggaag tataggtatc actacaccta gctgtatttt catattttaa aaaaaacgcc
    11581 taagaattgc tattgaatga ggcggagaga tggctcagca gtcacaagta cttgtagagg
    11641 actggggttt ggttactagc acctcaaaga tggcttacaa ttatctgtag ctccaattcc
    11701 agaggatctg tgtggtaaag ctgcatgtat atgggcatgc aggtaaaact gctaaacata
    11761 taaaaatagc tcagtcttaa gagaattggt cgacctcgag ggggggcccg gtacccaatt
    11821 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
    11881 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    11941 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    12001 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    12061 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    12121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    12181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    12241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    12301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    12361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    12421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtg
//
