LOCUS       sequence-125071-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 driven SpCas9 sgRNA expression for TS4 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pLKO1-puro-U6-sgRNA-TS4
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(24..507)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(60..78)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    556..673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(722..797)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
       61 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
      121 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
      181 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
      241 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
      301 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
      361 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
      421 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
      481 ccttggaaaa ggcgcaaccc caaccccctc gagccgcggc caaagtggat ctctgctgtc
      541 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
      601 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
      661 ttttctttta aaattgtgga tgaatactgc catttgtctc gaggtcgaga attcaaaaaa
      721 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
      781 atttctagct ctaaaacttc ttcttctgct cggactccgg tgtttcgtcc tttccacaag
      841 atatataaag ccaagaaatc gaaat
//
