LOCUS       sequence-125074-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA damage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Yang KS, Kohler RH, Landon M, Giedt R, Weissleder R
  TITLE     Single cell resolution in vivo imaging of DNA damage following PARP 
            inhibition.
  JOURNAL   Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129.
  PUBMED    25984718
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-05-18"; volume: "5:10129. doi"; pages: " 
            10.1038/srep10129"
COMMENT     Sequence Label: Apple-53BP1trunc
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             128..835
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     541..560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta ctagaggatc gctagcgcta ccgggatagc
      121 tagcaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg
      181 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      241 cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc
      301 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggtctacat
      361 taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg
      421 ggagcgcgtg atgaacttcg aggacggcgg cattattcac gttaaccagg actcctccct
      481 gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      541 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc gaggagcgga tgtaccccga
      601 ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg gcggccacta
      661 cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
      721 catcgtcgac atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca
      781 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggaga
      841 agaagagttt gatatgcctc agcctccaca tggccatgtc ttacatcgtc acatgagaac
      901 aatccgggaa gtacgcacac ttgtcactcg tgtcattaca gatgtgtatt atgtggatgg
      961 aacagaagta gaaagaaaag taactgagga gactgaagag ccaattgtag agtgtcagga
     1021 gtgtgaaact gaagtttccc cttcacagac tgggggctcc tcaggtgacc tgggggatat
     1081 cagctccttc tcctccaagg catccagctt acaccgcaca tcaagtggga ca
//
