LOCUS       sequence-125083-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 driven SpCas9 sgRNA expression for KANK3 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pLKO1-puro-U6-sgRNA-KANK3
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        53..128
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    177..294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        343..826
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     772..790
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tttatatatc ttgtggaaag gacgaaacac cggcatgggt gatgtcaatg ccgttttaga
       61 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      121 gtcggtgctt tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
      181 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      241 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      301 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
      361 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
      421 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
      481 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
      541 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
      601 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
      661 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
      721 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
      781 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
      841 cctctctccc c
//
