LOCUS       sequence-125084-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  8x Target 2 0gap with SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-321
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    61..195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(85..104)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     139..158
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      215..349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      121 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      181 tgcaataaac aagttagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
      241 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
      301 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggac tatggttgct
      361 gactaattga gatgcggatc cacccctaca cgacgtcccc atcttcggct cctcactcta
      421 gatccacccc taacgacgtc cccatcttcg gctcctcact ctagatccac ccctacacga
      481 cgtccccatc ttcggctcct cactctagat ccacccctac acgacgtccc catcttcggc
      541 tcctcactct agatccaccc ctacacgacg tccccatctt cggctcctca ctctagatcc
      601 acccctaacg acgtccccat cttcggctcc tcactctaga tccaccccta cacgacgtcc
      661 ccatcttcgg ctcctcactc tagatccacc cctacacgac gtccccatct tcggctcctc
      721 actctagatc cgctggcacg acagg
//
