LOCUS       sequence-125088-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Maitra M, Cano CA, Garcia CK
  TITLE     Mutant surfactant A2 proteins associated with familial pulmonary 
            fibrosis and lung cancer induce TGF-beta1 secretion.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21064-9. doi: 
            10.1073/pnas.1217069110. Epub 2012 Dec 5.
  PUBMED    23223528
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217069110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-18- 18"; volume: "109"; issue: "51"; pages: "21064-9"
COMMENT     Sequence Label: pLenti-SPA2G231V
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(16..63)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(112..441)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      126..261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(180..199)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     426..446
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(483..524)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    577..601
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
       61 aacacggtgc tgatcagatc cgaaaatgga tatacaagct cccgggagct ttttgcaaaa
      121 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg cagaggcggc
      181 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      241 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      301 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      361 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      421 tccacaccct aactgacaca cattccacag aattaattcc aaactcatta ctaaccggta
      481 cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc
      541 tctagactcg agcggccgcc actgtgctgg atatcaacca ctttgtacaa gaaagctggg
      601 tcggcgcgcc cacccttagt tttttgacac cagaccaact ggtaatggta gcgaccggcg
      661 ctcagctgta attccgccga tactgacggg ctccaggagt cgtctccacc aatccccata
      721 tggaaaccgt cgatattcag ccatgtgcct tcttccgcgt gcagcagatg gcgatggctg
      781 gtttccatca gttgctgttg actgtagcgg ctgatgttga actggaagtc gccgcgccac
      841 tggtgtgggc cataattcaa ttcgcgcgtc ccgcagcgca gaaccgtttt tcgctcggga
      901 agacgtacgg ggggtataca tgtcttgaca atgggcaaat cccagccggt caaaacaggc
      961 gggcaa
//
