LOCUS       sequence-125093-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Jones SA, Shim SH, He J, Zhuang X
  TITLE     Fast, three-dimensional super-resolution imaging of live cells.
  JOURNAL   Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.
  PUBMED    21552254
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "499-508. Epub 
            2011 May 8"
COMMENT     Sequence Label: pCLC-mEos2
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    134..255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(145..164)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     199..218
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(377..1057)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
       61 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
      121 acaatttacg ccttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      181 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      241 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      301 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      361 ctagagtcgc ggccgcttat cgtctggcat tgtcaggcaa tccagaatga gcaacagcat
      421 gctcatacag cttaaccttg ttgtaatctt tgtcatggct taaaatctca atgcagtggt
      481 ccacaaagtg gtagcctggt aacttgacac ccttctcctt agctttgtaa gtagttctga
      541 agtcacatcg gtaatgggca tttccttcaa gcaacaaagc catatgaata tcacccgtca
      601 gcactccatc acgcacatac attttctcag tggagggctc ccatttcagc gtcttcttct
      661 gcataactgg accattggcg ggaaagttgg taccataaaa tcgaacttta ttatagaaag
      721 tgtccccttc cattgttatg tcgtttctgg caatgcaaat gcccccgtct tcgaaagtca
      781 agcttcgttc ccacgaatac cccttaggaa acgactgctt aaaatagtct tgtatgttgt
      841 ctggatattt ggcgaatacc ctgttgccgt aatggaatgc agtggtcagg atatcaaagg
      901 caaaaggcag aggtccgccc tctttgactt caagatccat actctgtttt ccctcaaaag
      961 gcttgcctgt accatctccg tcgatcacaa agtggtgccc gtttacgttg ccttccatac
     1021 ggagtttgat cttcatgtct ggcttaatcg cactcatg
//
