LOCUS       sequence-125094-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Jones SA, Shim SH, He J, Zhuang X
  TITLE     Fast, three-dimensional super-resolution imaging of live cells.
  JOURNAL   Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.
  PUBMED    21552254
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "499-508. Epub 
            2011 May 8"
COMMENT     Sequence Label: pSNAP-CLC-EYFP
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(38..751,752..754,755..757))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     432..451
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtggtatggc tgattatgat ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      241 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      301 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      361 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      421 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      481 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
      541 cgaagcactg caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct
      601 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      661 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      721 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt gggtgcacca
      781 ggggggcctg cttcagggag atgaggactg agcgcatgcg agagacatct ttggcctgtt
      841 tgctggactt ggggttaaag tcacacaggc gggccacccg ctcccactca gtgcctgggg
      901 atgactcgtc aatgtcgttt acaaaggctt cttctgccgc cctgttgttt gcctttgtct
      961 tctgtagctg ctcgtcctgc ctcgcatacc actcttccag ctcctttatc gc
//
