LOCUS       sequence-125095-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Jones SA, Shim SH, He J, Zhuang X
  TITLE     Fast, three-dimensional super-resolution imaging of live cells.
  JOURNAL   Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.
  PUBMED    21552254
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "499-508. Epub 
            2011 May 8"
COMMENT     Sequence Label: pSNAP-CLC-EYFP
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..613
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgaggatg gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa
      121 gctggaactg tctgggtgcg aacagggcct gcacgagatc aagctgctgg gcaaaggaac
      181 atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc
      241 actgatgcag gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga
      301 gttccctgtg ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt
      361 gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc atcagctacc agcagctggc
      421 cgccctggcc ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc
      481 cgtgcccatt ctgatcccct gccaccgggt ggtgtctagc tctggcgccg tggggggcta
      541 cgagggcggg ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa
      601 gcctgggctg ggtatgaatt ctgcagtcga cggtaccgcc accatggccg agttggatcc
      661 atttggcgat cccgccggtg cgcccggtgg ccccgctcta ggaaacgggg tggcgggcgc
      721 cggcgaagaa gacccggccg cggccttctt ggcgcagcaa gagagtgaga ttgcgggcat
      781 cgagaacgat gaggccttcg ccatcctgga cggcggcgcc cccgggccgc agccacacgg
      841 cgagccgccg ggaggtccgg atgctgttga cggagtgatg aatggtgaat actaccagga
      901 gagcaatggt ccaacagaca gttacgcagc catttcagaa gtggatcgat tgcagtcaga
      961 gcctgaaagt atccgtaagt ggagagaaga gcagacagag cgcctggaag ccctcgatgc
     1021 caattctcgg aagcaagaag cggagtggaa agaaaaggcg ataaaggagc tggaagagtg
     1081 gtatgcgagg caggacgagc agct
//
