LOCUS       sequence-125099-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 driven SpCas9 sgRNA expression for TGM2 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pLKO1-puro-U6-sgRNA-TGM2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        54..129
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    178..295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        344..854
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     773..791
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(876..895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tttatatatc ttgtggaaag gacgaaacac cggcctggac ccaacgcccc agggttttag
       61 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      121 agtcggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattca tccacaattt
      181 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      241 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      301 ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc
      361 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
      421 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
      481 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
      541 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
      601 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
      661 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
      721 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
      781 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
      841 acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc
      901 gcctcgccac ccgcgacgac gtccccaggg
//
