LOCUS       sequence-125101-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 driven SpCas9 sgRNA expression for PLXNB2 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pLKO1-puro-U6-sgRNA-PLXNB2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..508)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(61..79)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    557..674
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(723..798)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(878..897)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gggagagagg tcggtgattc ggtcaacgag ggagccgact gccgacgtgc gctccggagg
       61 cttgcagaat gcggaacacc gcgcgggcag gaacagggcc cacactaccg ccccacaccc
      121 cgcctcccgc accgcccctt cccggccgct gctctcggcg cgccctgctg agcagccgct
      181 attggccaca gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc
      241 gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa
      301 ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc ggggggccca caagggtagc
      361 ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc
      421 gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct
      481 gccttggaaa aggcgcaacc ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt
      541 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
      601 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
      661 cttttctttt aaaattgtgg atgaatactg ccatttgtct cgaggtcgag aattcaaaaa
      721 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
      781 tatttctagc tctaaaaccc atgccctagg ttgggccccg gtgtttcgtc ctttccacaa
      841 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
      901 tttaaaacat aattttaaaa ctgcaa
//
