LOCUS       sequence-125107-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor pair 2 V2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-323
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    189..310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(226..245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     280..299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(342..491)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(604..615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
       61 agggattttg gtcatggcta gttaattaac atttccatac cacatttgta gaggttttac
      121 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      181 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      301 atgtatctta tcatgtctgg ccagctagct taacaattaa ttaacatatc gagatcgaaa
      361 tcgtccagag catcggagcc gagcatgtcc agatcaaagt catccaatgc gtcgcttccc
      421 aacatatcca ggtcaaagtc atcgagggca tcagaaccca gcatgtcgag atcgaaatcg
      481 tccagcgcgt cggcgcgccc tccgctgccc ttctcggtca tggtgcaatt atggacgaca
      541 acctggttgg caagcaaaaa ctgatgatca gaatcatcag ataaagtaat cccataataa
      601 tcgtcttcct tcaattcttg taactcgaaa taaaatccgc ggcactcacg tgcaaaagca
      661 gcggcgggag caggcctgaa ttttttagag ccggcacact tcgaaagaac gttaagcaaa
      721 acggatccgc ctccggaccc gccaccagat ccacctccgg acccacctcc tgagcctcca
      781 cctgatcc
//
