LOCUS       sequence-125110-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9 Op x6 -- GFP-IRES-NTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-352
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        133..164
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      191..200
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(197..199,200..202,203..715)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(242..263)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(503..522)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcgctagct gtagatggag aaatgtagat
       61 ggagaaatgt agatggagaa atgtagatgg agaaatgtag atggagaaat gtagatggag
      121 aagcttagac actagagggt atataatgga agctcgactt ccagcttggc aatccggtac
      181 tgttggtaaa gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      241 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      301 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      361 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      421 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      481 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      541 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      601 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      661 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      721 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      781 gctgcccg
//
