LOCUS       sequence-125114-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-338
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             89..109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccttttttag gtgttgtgaa aaccaccgct aattcaaagc aaccggtgat atcaaggatc
       61 catggactac aaagacgatg acgacaagcc taagaagaaa cggaaggtgt ccagacctgg
      121 ggagaggcca ttccagtgtc ggatctgtat gagaaatttt agcactatgg ctgtactgag
      181 gagacataca agaacccata caggcgagaa gccctttcag tgcaggatct gcatgcgaaa
      241 cttttctcgc agagaagtgc ttgaaaacca cctgcggact cataccggcg aaaaaccatt
      301 tcagtgtcga atttgcatgc gcaactttag ccagacagtg aacttggata gacaccttaa
      361 gactcatctg aggcagaagg acggggagag acctttccag tgcagaatct gcatgagaaa
      421 cttttctaag aaagatcatc tgcaccgcca tacccgcacc cacaccggcg aaaaaccctt
      481 ccagtgccgc atctgtatgc gaaattttag ccagaggcca catcttacaa atcaccttag
      541 aacacacact ggagaaaaac catttcagtg tcggatctgc atgcgcaact tctccgtggg
      601 cgccagcctc aaacgccacc tgaaaaccca cctgagggga tctgggggcg gatcaggtgg
      661 aggctcagga ggtgggtccg gaggtggatc tggtggcggg tccggagg
//
