LOCUS       sequence-125122-        1291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to knockdown endogenous RAB14 expression levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
  TITLE     MIR144 and MIR451 regulate human erythropoiesis via RAB14.
  JOURNAL   Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
  PUBMED    25312678
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Br J 
            Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164."
COMMENT     Sequence Label: RAB14 shRNA
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..265
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     17..37
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     188..207
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    361..478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        527..1037
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     956..974
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1053..1062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1104..1125)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccggagg agcgatttag ggctgttact cgagtaacag
      301 ccctaaatcg ctcctttttt aattctcgac ctcgagacaa atggcagtat tcatccacaa
      361 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      421 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      481 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
      541 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      601 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      661 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      721 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      781 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
      841 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
      901 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
      961 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     1021 ccgacctctc tccccagggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     1081 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1141 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1201 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1261 gcgtgcagtg cttcagccgc taccccgacc a
//
