LOCUS       sequence-125125-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-SaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagagctctc tggctaacta ccggtgccac catggcccca aagaagaagc ggaaggtcgg
       61 tatccacgga gtcccagcag ccaagcggaa ctacatcctg ggcctggaca tcggcatcac
      121 cagcgtgggc tacggcatca tcgactacga gacacgggac gtgatcgatg ccggcgtgcg
      181 gctgttcaaa gaggccaacg tggaaaacaa cgagggcagg cggagcaaga gaggcgccag
      241 aaggctgaag cggcggaggc ggcatagaat ccagagagtg aagaagctgc tgttcgacta
      301 caacctgctg accgaccaca gcgagctgag cggcatcaac ccctacgagg ccagagtgaa
      361 gggcctgagc cagaagctga gcgaggaaga gttctctgcc gccctgctgc acctggccaa
      421 gagaagaggc gtgcacaacg tgaacgaggt ggaagaggac accggcaacg agctgtccac
      481 caaagagcag atcagccgga acagcaaggc cctggaagag aaatacgtgg ccgaactgca
      541 gctggaacgg ctgaagaaag acggcgaagt gcggggcagc atcaacagat tcaagaccag
      601 cgactacgtg aaagaagcca aacagctgct gaaggtgcag aaggcctacc accagctgga
      661 ccagagcttc atcgacacct acatcgacct gctggaaacc cggcggacct actatgaggg
      721 acctggcgag ggcagcccct tcggctggaa ggacatcaaa gaatggtacg agatgctgat
      781 gggccactgc acctacttcc ccgaggaact gcggagcgtg aagtacgcct acaacgccga
      841 cctgtacaac gccctgaacg acctgaacaa tctcgtgatc accagggacg agaacgagaa
      901 gctggaatat tacgagaagt tccagatcat cgagaacgtg ttcaagcaga
//
