LOCUS       sequence-125126-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-SaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagatgtgtc gaagatggac agtcctgtgc tgatatgcat ggcatgcaag aggcactcgt
       61 cgttcaggaa ggtatccaga atctcgttca attccggggt caggggtgag tccaggttca
      121 gatcctcggt catggactca agtgtggttg tcagctcatc cagatggccc cttgggggcg
      181 gatgggaaag gtccatttgg ccacagattg cagcctcttc cttctgggga atcacagtat
      241 cggccatctc ccgaagggct ttgacagcct ggctcgtctc ttcatcggga tcctccaaca
      301 ggtgactggc ctcgggagtc actgcgggcg ctggatccag tggctgaggg actggtgccg
      361 gggtcagtga cccgactggc tcatgtactg gaccggttgg tgttggtgcg aggctggcgg
      421 ggagtgggcg gttggcccat ggacttcctg gaggatgaaa tggccggatt cgttttggct
      481 ggcacacctc gcggccctca aacacgtcac taatagcgga gccggcctca ggcttcggca
      541 aaaacatccc ttccctggaa tcccggctgc cgctgccaga gcccagcaag gctgagaaat
      601 ccatatcggc gatagagctg aagtcctcgt cgccagacag cagtccatta ggcaggcctg
      661 gtgctcccag aggggcagga gctggatcag gaggcctctg agcgcctgtc acgagccggg
      721 tgatggcctc ggggtattcc atcagcatgg gctcggtggt gtgaggggcc acagggatgc
      781 cctggttcag cagctgctgg aactcgctgt tgtccacgct ggccaggtcg gtgaacacgg
      841 caggatcggt
//
