LOCUS       sequence-125129-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/staphylococcal temperature-sensitive plasmid for allelic 
            exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Monk IR, Shah IM, Xu M, Tan MW, Foster TJ
  TITLE     Transforming the untransformable: application of direct 
            transformation to manipulate genetically Staphylococcus aureus and 
            Staphylococcus epidermidis.
  JOURNAL   MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. 
            Print 2012.
  PUBMED    22434850
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2012-03-20"; volume: "3(2). pii: e00277-11. doi"; pages: " 
            10.1128/mBio.00277-11. Print 2012"
COMMENT     Sequence Label: pIMAY
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..57)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(42..58)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(87..105)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
       61 gggggcccgg tacccagctt ttgttccctt tagtgagggt taattatccc attatgcttt
      121 ggcagtttat tcttgacatg tagtgagggg gctggtataa tcacatacgg ccgataaagc
      181 aagcatataa tattgcgttt catctttaga agcgaatttc gccaatatta taattatcaa
      241 agagaggggt ggcaaacggt atttggcatt attaggttaa aaaatgtaga aggagagtga
      301 aacccatgaa ctttaataaa attgatttag acaattggaa gagaaaagag atatttaatc
      361 attatttgaa ccaacaaacg acttttagta taaccacaga aattgatatt agtgttttat
      421 accgaaacat aaaacaagaa ggatataaat tttaccctgc atttattttc ttagtgacaa
      481 gggtgataaa ctcaaataca gcttttagaa ctggttacaa tagcgacgga gagttaggtt
      541 attgggataa gttagagcca ctttatacaa tttttgatgg tgtatctaaa acattctctg
      601 gtatttggac tcctgtaaag aatgacttca aagagtttta tgatttatac ctttctgatg
      661 tagagaaata taatggttcg gggaaattgt ttcccaaaac acctatacct gaaaatgctt
      721 tttctctttc tattattcca tggacttca
//
