LOCUS       sequence-125130-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/staphylococcal temperature-sensitive plasmid for allelic 
            exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Monk IR, Shah IM, Xu M, Tan MW, Foster TJ
  TITLE     Transforming the untransformable: application of direct 
            transformation to manipulate genetically Staphylococcus aureus and 
            Staphylococcus epidermidis.
  JOURNAL   MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. 
            Print 2012.
  PUBMED    22434850
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2012-03-20"; volume: "3(2). pii: e00277-11. doi"; pages: " 
            10.1128/mBio.00277-11. Print 2012"
COMMENT     Sequence Label: pIMAY
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..65)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(49..65)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(97..116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(98..116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(489..506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(703..722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
       61 ctagagcggc cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacgac
      121 gtcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
      181 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
      241 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
      301 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
      361 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
      421 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
      481 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
      541 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
      601 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
      661 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      721 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtt
//
