LOCUS       sequence-125143-        9370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a targeting vector, to target the endogenous Gapdh CPG island with 
            Snrp-GFP-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.08"; journalName: "Cell"; date: "2015-09-24- 
            24"; volume: "163"; issue: "1"; pages: "218-29"
COMMENT     Sequence Label: GAPDH-CPG-TV
FEATURES             Location/Qualifiers
     source          1..9370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1628..1630,1631..1633,1634..2347)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1628..1630,1631..1633,1634..2344)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1673..1694)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1934..1953)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2281..2302
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2437..2456)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2502..2557
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2556..2575)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    2918..2951
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3012..3508
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3047..3070)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3441..3460
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3548..4147
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3548..3567)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4044..4064
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    4704..4737
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5803..5822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5804..5822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5829..5846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5829..5845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5838..5860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5986..6414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6073..6092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6283..6304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6758..7552
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6812..6831)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7422..7441
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7570..7638,7639..8430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7788..7807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8601..9189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9090..9109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9343..9360
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggagccga gaggaatgag gttagtcaca agctaggaag aaggaaggcc taagcaagat
      361 ttcataacgg cggttcattc atttccttcc cgtttgcaac atggcggcgg ccagttaccc
      421 taaaggaagc ccaagcctgg gagatgatgc gcaaaggtat gcacctcaca acgccatgcc
      481 accgaccccg aggaaggggg agatggggga ggggcttccc tcctggcacg tgactcctca
      541 gggcgcgaaa gtaaagaaag aagccccgga ctggggatta cgggatgggt ctgaacgggc
      601 gcgtggggtt gttcctaata cttaagactc cgggccgctc atccccgcaa aggcggagtt
      661 accagaggtg cagcagctgg gggccgcaag tgcgagccat cgccaggtcc gagcgctgac
      721 cttgaggtct ccttgggcgc ttcggccacc tcatccacct ccccacagta ctgcagagcc
      781 cccgggtcag caagacctgc gtaggcaacg gagcagggcc ccgccctgga actcacccgt
      841 tcacaccgac cttcaccatt ttgtctacgg gacgaggctg caggagaaga aaatgagatt
      901 agcgtggccc gaaggacacc aggcagtccc ctcccccacc ccgcctccat tcccgccgtg
      961 cgccccatca cgtcctccat catccccccc ctgcaatgcg gagcctgggt ccggcttgca
     1021 cacttcgcac cagcatccct agacccgtac agtgcccact ccccttccca gtttccgact
     1081 gtccccggcc tcctgcggcc cactccgcga ttttcacctg gcactgcaca agaagatgcg
     1141 gccgtctctg gaacagggag gagcagagag caccagggag ggctgcagtc cgtatttata
     1201 ggaacccgga tggtgggggg gagctgatga cgcgcgcccc gcctcccgcc ctgcttatcc
     1261 agtcctagct caagggcgca gaggcctgag ctacgtgcac ccgtaaagcc gcgagtagct
     1321 gggcctctct ccaattgacg ctcaaatttc cgcagtagga atgctcaagc attccttttg
     1381 gtagctgcct tttggcagga cattccggtc agagggacag agacccctgc attgcggcaa
     1441 aaatgtgcgc atgtgcagcc attgcctggg acgcatgcgt agggagccgc gcgacaaacc
     1501 tgagccattg cggcaagact agcgcagaga ggagagggag ccggagatgc cagacgcttg
     1561 gttctgagga gtgatttgca acgcaatgga gcgaggaagg tcagctgggc ttgtggattc
     1621 tgctagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1681 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1741 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1801 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1861 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1921 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1981 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2041 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2101 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2161 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2221 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2281 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2341 caagtaaacg cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     2401 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     2461 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     2521 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2581 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     2641 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     2701 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     2761 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     2821 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     2881 atcccttaat taacgatcat attcaataac ccttaatata acttcgtata atgtatgcta
     2941 tacgaagtta ttaggtctga agaggagttt acgtccagcc aagcttagga tctcgacctc
     3001 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     3061 agccccgctg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     3121 accggtagcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     3181 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     3241 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     3301 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagcagc
     3361 tgggaagggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     3421 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     3481 ctcctcttcc tcatctccgg gcctttcgac ctgcatccat ctagatctcg agcagctgaa
     3541 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3601 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3661 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3721 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3781 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3841 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3901 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3961 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     4021 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     4081 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     4141 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     4201 atcgatgata tcagatcccc gggatgcaga aattgatgat ctattaaaca ataaagatgt
     4261 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
     4321 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
     4381 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
     4441 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
     4501 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
     4561 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
     4621 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct
     4681 ggtcgagatc cggaaccctt aatataactt cgtataatgt atgctatacg aagttattag
     4741 gtccctcgaa gaggttcact aggcgcgcct ttttgaaatg tgcacgcacc aagcgtgtgg
     4801 gctccgaact gataggggga ggggagctga gattgccccg ctcaggaggt gaccgggtga
     4861 tggttcccag gataggactc agggaataca gactatgaac ttaaggacga ggagtccttg
     4921 gagtgtgcac caaggacatc caggacccag agacctgaat gctgcttccc gagtaaaatg
     4981 ggagaacagg ggaaatggag aagtgtgggt ctccctgcca ctgcatcatc gaacctctcc
     5041 ccattattga aaaggctgcg gaaaagttga ggatcttctc ctcgttgttc ttcggtagtg
     5101 acacagcaac cccccccccc gaaaaaaaaa agagtggaag aaaatgccag ggtgggggca
     5161 cttttcctcc cgcgggagcc ctcaggagcc ccatttccct atctgccccc ccccccctcc
     5221 tggcttctgt ctttggccag aggcctggct tgtgtaccgc gctgtgaggg caagggggtg
     5281 gccaggaaga cgcttgaaaa ggagattgct acgccatagg tcaggatgcc ctgcagcctg
     5341 gaaacctgat aattgagact tacaaacact ctcctgagtt tacctgatga acctaagctg
     5401 ggaccccggg gtaagggcag catccctctt aagggttgaa ttggaggagg ctcagaggcc
     5461 tcctatagta tccctcctcg gaacctgaga gctgccccgc ccaggctagg agacactgcc
     5521 cctttcaccc actgtagccc cagggtggag agttgggacg tgagggcgga agtccctccc
     5581 catccaggga cgtgctgact ggccctggag ccagggactc tccttttttg gctgccctcg
     5641 tggacacagg gtgagatcag tgaggggaga acacaggaga ggaaatatta tacccctgag
     5701 ccaggacagg gaagccccta gcggccggcc aataagggcg aattctgcag atatccatca
     5761 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat
     5821 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5881 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5941 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     6001 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6061 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6121 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6181 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6241 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6301 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6361 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6421 aaattcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
     6481 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
     6541 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
     6601 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
     6661 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
     6721 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6781 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6841 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6901 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6961 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7021 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat cccaccttgc
     7081 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7141 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7201 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7261 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7321 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7381 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7441 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7501 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattgaaaa
     7561 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7801 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8101 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8401 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9361 gaagcggaag
//
