LOCUS       sequence-125156-        2316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector 
            with DS3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS3-aurJ
FEATURES             Location/Qualifiers
     source          1..2316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..688
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     558..581
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(2106..2124)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcagatctca gttcgagttt atcattatca atactgccat ttcaaagaat acgtaaataa
       61 ttaatagtag tgattttcct aactttattt agtcaaaaaa ttagcctttt aattctgctg
      121 taacccgtac atgcccaaaa tagggggcgg gttacacaga atatataaca tcgtaggtgt
      181 ctgggtgaac agtttattcc tggcatccac taaatataat ggagcccgct ttttaagctg
      241 gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc aaccatcagt
      301 tcataggtcc attctcttag cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg
      361 gacacaacct caatggagtg atgcaacctg cctggagtaa atgatgacac aaggcaattg
      421 acccacgcat gtatctatct cattttctta caccttctat taccttctgc tctctctgat
      481 ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg aaattattcc cctacttgac
      541 taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact
      601 tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacca agaacttagt
      661 ttcgaataaa cacacataaa caaacaaaaa gcttaaaatg ggttcaatta gctctccttc
      721 actaataatc gatttggcaa acgcggtgtc atcagcagcg aaaaacttgg atacacagtt
      781 gcagtcccaa ggttttcctc aaccttcatt cgaagctgac ggaccaactt acgttgtacc
      841 taaggatgca ccaaaagctg ctcacgaagc aagagtagcc actgccgaag ctgcattaaa
      901 actgttcaac ctagtttcag gtccaagcga attgttacca aatatgacag catcttacca
      961 cacaattttt gctttgcaat ggctccatca tttcgatgtc ttttctcaca ttcctctaga
     1021 tggcagttta tcttacgaaa agctggccac taaagccaat gtacctgaat ctttactgaa
     1081 aagtgttgcc aggatggcta tgacctctaa tatccttgct gaacctacta ctggccaagt
     1141 tgctcattcc gctaactctg cgatgtttgt gaagtttcca aatatgcgtg attgggctag
     1201 ttatatgttt accgcatcaa taccaactgc tgccgctatg gttcaagcca cagaaaagtg
     1261 gccaggctcc gtcaaaaaga cagagacagc atacaatata gctttcaatc atgatttgcc
     1321 atttttcgat cacttatctc aatctccagt gatgacaaaa caattctctg ggtatatgag
     1381 atccgtcaca gacggtcaag gaatggattt gtcccatttg gttaatggct ttgactgggc
     1441 atcccttcct gacaagtcac taatcgtgga catcggtgga tcagcgggac acgcaagcta
     1501 cgcccttgct gctgcttacc cacatcttcg ttttgaagta caggatctag atacagtcgt
     1561 taatggtgaa aaagcagcaa aagagcatga ggaagccgtt tcaaaacatg tcattgggac
     1621 cgacaataga gttactttta aagctcataa ctttttcgag gctcaaccta caaaggacgc
     1681 aaccgtgtat atgctgcgaa tgatcatcca tgattggcca gatgcggaag ccaagaccat
     1741 attaggtaat cttgttccag cattggaatc tgccaaagct actctgttga ttatggacac
     1801 agtcctacca tctccagggt caattccttc tgtaagggag agagtgatca gaacgagaga
     1861 tttgactatg agacaagtat tcaacgcaaa ggaaagaggt gttgatgatt gggaagctat
     1921 cttaagagaa acggacagta gattgacatt gaaaaatctc agacaaccag agggttctaa
     1981 catgtgttta ctcactatta gtttacaaga tgactaaccg cggcacgtcc gacggcggcc
     2041 cgacgggtcc gaggcctcgg agatccgtcc cccttttcct ttgtcgatat catgtaatta
     2101 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
     2161 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
     2221 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
     2281 attatactga aaaccttgct tgagaaggtt ttggga
//
