LOCUS       sequence-125161-       11313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector 
            with DS1U selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS1U-PKS12
FEATURES             Location/Qualifiers
     source          1..11313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(133..721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(213..232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(892..1683,1684..1752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1515..1534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1753..1857)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1894..2397
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2438..2456
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2494..2516)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2616..2635
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2674..2707
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2844..3647)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    3939..3972
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4285..4519
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     protein_bind    9326..9337
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     terminator      10948..11135
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(11003..11027)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(11275..11292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
       61 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      541 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
      601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      661 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      721 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      781 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      841 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      901 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
      961 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1021 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1081 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1141 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1201 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1261 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1321 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1381 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1441 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1501 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1561 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1621 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1681 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1741 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1861 atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat
     1921 ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga
     1981 aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact
     2041 accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg
     2101 tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg
     2161 tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt
     2221 tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct
     2281 ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc
     2341 ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt
     2401 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2461 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2521 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2581 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2641 agtgcaccat aacagctttc gacaaccctt aatataactt cgtataatgt atgctatacg
     2701 aagttattag gtctagagat cccaatacaa cagatcacgt gatcttttgt aagatgaagt
     2761 tgaagtgagt gttgcaccgt gccaatgcag gtggctatta gattaaatat gtgatttgtt
     2821 ctattaagtt tcctgtataa gctctagttt tgctggccgc atcttctcaa atatgcttcc
     2881 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     2941 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     3001 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     3061 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     3121 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     3181 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     3241 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     3301 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     3361 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     3421 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     3481 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     3541 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     3601 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacataat tattctcgac
     3661 tcaacttcaa tccgttttct ttcttcttat tgcgctaatt gtctgttaga cacttttatg
     3721 agacttcctt tttgtcggcg gctatttctc aatatactcc taattaatac atttccgtgc
     3781 aaaggtacta actagctaaa cggagactct agagcttgca tgcctgcagt actgtatgta
     3841 catacagtac tgtatgtaca tacagtactg caggtcggaa cgctgtatat atatcagatc
     3901 tgtatatata tacagacgag ctttcgagaa cccttaatat aacttcgtat aatgtatgct
     3961 atacgaagtt attaggtgat atcagatcca ctagtggcct atgcacccaa ttgcgcaact
     4021 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctgatt tgcccgggca
     4081 gttcaggctc atcaggcgcg ccatgcaggg atatcagatc tccattaccg acatttgggc
     4141 gctatacgtg catatgttca tgtatgtatc tgtatttaaa acacttttgt attatttttc
     4201 ctcatatatg tgtataggtt tatacggatg atttaattat tacttcacca ccctttattt
     4261 caggctgata tcttagcctt gttactagtt agaaaaagac atttttgctg tcagtcactg
     4321 tcaagagatt cttttgctgg catttcttct agaagcaaaa agagcgatgc gtcttttccg
     4381 ctgaaccgtt ccagcaaaaa agactaccaa cgcaatatgg attgtcagaa tcatataaaa
     4441 gagaagcaaa taactccttg tcttgtatca attgcattat aatatcttct tgttagtgca
     4501 atatcatata gaagtcatcg aaatagatat taagaaaaac aaactgtaca atcaatcaat
     4561 caatcatcac ataaagctta tggaggtatt cgtttttggg gaccaaagca cacgctttgc
     4621 ccctccactg aaagacctac tcctcaaagg caacagtcct tacttgacac attttgttaa
     4681 acaagttcac gcacttctta gaagggagat atcatccttg ccggcagttc aacagaagct
     4741 atttccaaac tttgccgaca ttcaggaact cgtctccaag tcagattggg gcagtggtaa
     4801 ccctgctttg acaagcgctt tagcatgctt ttaccatctt tgcagtttca ttcactttta
     4861 cgatggacaa ggtcgtacct ttccttcgga gaacagtcgc attattggac tttgcgttgg
     4921 ttcactcgct gctgctgctg tcagttgctc cacatcactg agtgaactgg tatcagctgg
     4981 tgtagatgct gttcgtgtgg cattgcacgt cggactacgg gtatggcgaa ctacctccct
     5041 tttcgatgta ccagacaggc cctccgccac ttggtccata attgtgcccg aggcagtact
     5101 accaagagaa tctgcgcaag accgacttga ctcattcatc attgaaatgg gacttgctcg
     5161 atcgtcagtt ccttacatca gctcggtcgc acatcacaac atgaccatca gtggtccacc
     5221 atccgtcctc gaaaagttca ttcacagtat atcaacatca ccgaaagatt ctcttccagt
     5281 gccgatctat gctccgtacc acgccagcca tctttacagc atggatgatg tagacgaggt
     5341 ccttagcctg tctgcacctt cttttgcatc agagtccatc attccactca tttcaagctc
     5401 ctcgggtgac gagttacagc cactcaagta tgcagatcta ctccgctgct gtgttagtga
     5461 tatgctcata cagccactgg atcttaccaa ggtctcacaa gcagtggccc agcttctcga
     5521 ggttagctca tctacacgtg ccataataaa gcctatagca accagcgtct ccaacagtct
     5581 agtgtctgct ttggagccga cgctagcaga acgatgcgcc gtggacaaca gcatggggcc
     5641 caaagcctcg accagccact catcagcaga gacacaaacc gagtcatcaa gcaagaactc
     5701 caaaattgcg attgttgcta tgtctggtcg ctttccagac gcagctgact tgggtgaatt
     5761 ctgggatctt ctctacaaag gtcgcgatgt tcatcgacaa attcccgagg accgattcaa
     5821 cgcagagctc cattacgacg ctactgggcg acgtaagaac accagcaagg tcatgaatgg
     5881 ctgcttcatc aaggaaccag gactgttcga cgctaggttc ttcaacatgt ctccaaagga
     5941 agctgagcag tcggatcctg gccagcgaat ggccctcgaa acagcttacg aggcgcttga
     6001 gatggctggt atcgtaccag acagaacacc ttcgacacag agagatcgtg ttggtgtgtt
     6061 ctacggcatg actagcgatg attggagaga ggtcaacagt gggcagaatg tcgacactta
     6121 ttttattcct ggtggcaaca gagcgttcac tcctggtcga ctcaactact tcttcaagtt
     6181 cagtgggcct agcgctagtg ttgatacggc ttgctcctcc agtctcgctg ccctacactt
     6241 ggcttgcaat tccctctgga gaaatgattg cgatacagct attgcgggcg gaaccaatgt
     6301 catgactaac cctgacaact tcgctggttt ggaccgaggc cacttcctat caagaaccgg
     6361 caactgcaac acctttgacg atggagcaga cggatactgt cgagctgatg gcgtcggaac
     6421 catcatcctc aagcggctcg aggacgccga agctgacaat gaccctattc tcggtgtcat
     6481 tctgggcgct tacacaaacc actcagccga agcagtatcc atcactcgac cacatgccgg
     6541 agctcaagag tacatcttct ccaaactcct ccgtgagtcg ggcaccgatc cctacaacgt
     6601 tagctacatc gagatgcacg gcacaggcac tcaagccggc gacgcaaccg agatgacatc
     6661 cgtcctcaag acgtttgctc ctaccagcgg ctttggcggt cgattgcctc accaaaacct
     6721 tcacttgggt tcagtcaagg ccaatgtcgg gcacggtgaa tccgcatctg gtatcattgc
     6781 tctgatcaag acgctgctta tgatggagaa gaacatgatc ccgccgcatt gtggtatcaa
     6841 gacaaagatc aatcaccatt ttcctacgga tctcactcag cgcaatgtcc atatcgccaa
     6901 agttccgaca tcttggacaa gatctggtca agccaatcca cgcattgctt tcgtcaataa
     6961 cttctctgcc gctggtggta actctgctgt cctactgcaa gatgctcctc ggccatcggt
     7021 agtttcggat gttacagacc ctcgctcatc ccatgttgtc actatgtccg ctcgatcagc
     7081 agattccctc aggaagaacc tcgccaatct caaggagctt gtagaaggcc aaggtgactc
     7141 ggaggtcgac ttcctgagca agctgtccta cacaaccacc gccaggcgca tgcatcatca
     7201 attccgagct tcggtcacag cacagactcg tgaacagctg ctgaagggcc ttgattccgc
     7261 cattgaacgc caggatgtga agaggatccc cgctgccgcg ccctctgtcg gctttgtgtt
     7321 tagcggccaa ggcgcccaat accgtggtat gggcaaggag tactttacat ctttcacagc
     7381 cttccgctct gagatcatgt cttacgacag tatcgcccaa gcccaaggct tcccgtcaat
     7441 cctcccactg atccgaggag aggtggaagc tgactcgttg agtcctgttg agatccagct
     7501 gggtctcact tgcctgcaga tggcactggc caagctatgg aagtcattcg gtgttgagcc
     7561 aggctttgtt ctcggacaca gcttaggcca ctatgctgct ttacacgtcg ctggtgttct
     7621 gtccgccaat gataccattt acctcactgg catcagagcg cagctgctcg tggataagtg
     7681 ccaggcagga acccactcaa tgctggcagt aagggcatcc ttactccaga tccaacagtt
     7741 cctcgatgcc aacattcacg aggttgcatg tgtcaatggt ccacgagaag tcgtcatcag
     7801 tggacgcgtt gccgacattg accagctggt tggccttttg tcggctgaca acatcaaggc
     7861 gacccgcgtc aaggtgccat ttgccttcca ctcagcgcag gttgacccca ttctctccga
     7921 cttggataca gcggcgtcgc gcgtcacctt ccactccccc cagattcctg ttctttgtgc
     7981 ccttgacagc tctgtcatca gccctggaaa ccatggtgtc attggtcccc ttcatctaca
     8041 gcgacattgt cgtgagacag tcaactttga gggtgctcta catgctgcgg agcgcgagaa
     8101 gatcatcaac aagacatcaa ctctatggat cgagattggt ccccatgttg tctgctctac
     8161 cttcctcaag tccagccttg gtccaagcac acctactatc gcatcgcttc gccgaaatga
     8221 cgattgctgg aaggtgttgg ctgatggttt gagcagtctc tacagcagcg ggttgacaat
     8281 tgactggaac gagtatcatc gcgacttcaa ggcctctcac caggtacttc gtctgccttg
     8341 ttacagctgg gagcacaaga attactggat acagtacaag tacgattggt ccttgactaa
     8401 aggtgatcct ccaattgctc ctaacagctc ggttgaagca gtctcagctt tatcaacacc
     8461 ctcggtccag aagattctac aggagacttc ccttgatcag gtgttgacta tcgttgctga
     8521 gacagatctc gcgagccctc tattgtcaga ggttgcccaa ggtcatcggg tcaacggtgt
     8581 caaagtctgc acatcttccg tgtacgctga tgttggcttg acgctgggta agtacatttt
     8641 ggacaactac cgcaccgact tagagggtta tgcggtcgat gttcacggta ttgaggtcca
     8701 caagccactt cttctcaaag aagacatgaa cggaacgccc caggctacac cgttccgcat
     8761 cgaagtgcga tacccaatcc agagcaccac ggcgctgatg agcatctcca ccactggtcc
     8821 caacggtcag cacatcaagc atgctaactg cgaacttcga ctcgagcatc cgtcgcaatg
     8881 ggaagcggag tgggatcgcc aagcctacct catcaatcgc agcgtcaact accttctgca
     8941 gcgatcagca caaggtttgg acagcatgtt ggcaaccgga atggtctaca aggtcttctc
     9001 ctccctcgtc gactatgccg atggctacaa gggtctgcag gaggttgtct tgcacagcca
     9061 agagctcgag ggcacagcaa aagtgcgctt ccaaactccc tcgggaggtt tcgtctgcaa
     9121 tcccatgtgg attgacagct gtggtcagac gaccggcttc atgatgaact gtcatcagac
     9181 tacgcccaat gactacgtct acgtcaatca tggctggaag tcgatgagat tggccaaggc
     9241 gttccgtgaa gatggtacct atcggactta tatccggatg aggcccattg atagcaccaa
     9301 gttcgctggt gacttgtaca ttcttgatga ggatgacact gtggttggtg tttatggaga
     9361 cataacattc caaggtttgc cgcgacgagt cctcaacaca gtcttgccat ctgccaacgc
     9421 ggttccagtt gatgctccca tgggtcgacg ggacgtgcct ccatcaagaa tggatgtgcc
     9481 tcccgtcagg tccggtgaag gcccacccac ttcagcaccc acgcagcaag ctatcgctct
     9541 gccgttcgca gccgatacat ccatggactc ccgattgaga cctcttcttc gcatcttgtc
     9601 agaagagatc ggtctcggtc ttgacgttct ttcggacgat gaactcgact ttgcggacca
     9661 cggtgtcgac tcactcctct cattgaccat cactggtcgc atgcgtgagg aattgggtct
     9721 cgacgttgaa tctacagcat tcatgaactg tcccactttg ggcagcttta aattattcct
     9781 aggacttgtc gatcaggaca ataagagcag cagcggcagt gatggcagtg gtaggagcag
     9841 tccagcaccg ggtatcgagt ctggcgctac tacaccacct atgagcgaag aggaccagga
     9901 caagatagtc agcagtcact cgcttcacca gttccaagcc agttcgacgc ttctacaggg
     9961 cagtcccagt aaagctcgct cgactttgtt cttgctacca gatggctcgg gatctgccac
    10021 atcctacgct tcccttcccc cgatctctcc agacggagat gttgctgtct acgggttgaa
    10081 ctgcccatgg ctgaaggacg ctagttacct cgtcgagttt ggactcaagg gcttgacaga
    10141 gctctatgtc aacgagatac tccgtcgcaa gccacagggt ccttacaatt tgggaggatg
    10201 gtcagccggt ggcatttgcg cttatgaagc tgccctgatc ctcaccagag caggacacca
    10261 agtcgatcgc cttatcttga ttgactctcc caatcccgtt ggtcttgaga agctacctcc
    10321 tcgcttgtac gatttcctca attcgcagaa tgtctttgga tcagacaacc cgcacagcac
    10381 tgctggaaca agcgtcaaag ctccagaatg gcttcttgca catttcctgg ccttcattga
    10441 cgctctggat gcttatgtcg cagtgccttg ggactctggt ctagtcggtc tagcatcacc
    10501 gctccctgca ccgccgcaga catacatgct gtgggcagaa gacggagttt gcaaagactc
    10561 tgatagtgct cgtcccgagt accgtgacga tgacccacgc gagatgagat ggctgttgga
    10621 gaacagaaca aactttggtc cgaatggttg ggaggcgcta cttggtggta aagagggttt
    10681 gttcatggat cggattgcgg aagcgaatca ttttagtatg ttgaagagag gacggaatgc
    10741 ggaatatgtc tctgcattcc tggctcgggc cttggacaat tagccgcggc cgcaggccta
    10801 aattgatcta gagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt
    10861 gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt
    10921 agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt
    10981 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
    11041 cgaaaattct tgttcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
    11101 catgaggtcg ctcttattga ccacacctct accggcatgc ccatgggtta actgatcaat
    11161 gcatcctgca tggcgcgcct gatgagcctg aactgcccgg gcaaatcagc tgcattaatg
    11221 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11281 cactgactcg ctgcgctcgg tcgttcggct gcg
//
