LOCUS       sequence-125181-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal histidine-tagged DXH36 gene product upon 
            transfection into Rosetta 2 E. coli and induction with IPTG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Vaughn JP, Creacy SD, Routh ED, Joyner-Butt C, Jenkins GS, Pauli S, 
            Nagamine Y, Akman SA
  TITLE     The DEXH protein product of the DHX36 gene is the major source of 
            tetramolecular quadruplex G4-DNA resolving activity in HeLa cell 
            lysates.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38117-20. Epub 2005 Sep 7.
  PUBMED    16150737
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38117-20. 
            Epub 2005 Sep 7"
COMMENT     Sequence Label: TriEx-4 DHX36
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..86)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(93..125)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag gttaatcact
       61 tagtgatggt gatggtgatg gtggtgctcg agatcctcgg ggtcttccgg ggcgagttct
      121 ggctggcttg cacgtgtata cagctgtgcg gccgcaagct tcgaaccggt accgtcgacc
      181 tgcaggcgcg ccgagatctg agaattcgga tccacgagct cagatatcgt tgaggagaag
      241 cccggactcc gatccttttg gatccagtct ttactaccta ctgaaggctt ctaccttaca
      301 catgaaaatt gttcatgtcc cagggtttgg catccagcca aaatttaaac aatgatgaag
      361 aatggctgtc aaactggctt ttcagaccac ccctgaaaag ctgtcagctg taatatccat
      421 cctggaatcg tggcggaaag ttcctgggag ttgccttttc ctgtgttttg atcaagtcta
      481 taatagctga cagtactgca cagtctctgg atttagtgtc attccagtct acaggatgag
      541 gactttcaat cttctcttgc agaagaatat ctagttcctt tcttaattcc ttaacaagat
      601 gggcaattct tgctggagac tgaaatacaa tccactcatc tacagcaata gtttcctgat
      661 cgttatcctt ctggatggaa atgtcacctc caaaaaacaa gagacagtat ggggaaacct
      721 ctgtgcagtc atacaagtat atactgcttg ttctcatctt taggtgatag ataagccagt
      781 tgtagtgaaa gtctgtttgc tccacattaa cagatttagg atgaacagca accaggccat
      841 cggtttttgt gtaaactttt accatttttc tttttttacc caaatttagt cgaattttag
      901 caactttggg atataaacca gcacagatga cagctttaat tatcttctca ttatctgaat
      961 ttatattaga ttctggatct ttaggatttc tactgcttac aaatccagct ccaagaagat
     1021 gctcagcaaa ctgtcctttc atgttatgca gcatctgcag tgtgtttgaa gacagaaaat
     1081 attcccagca atagtccttt tcgtatctga a
//
